ArticleInternational journal of molecular sciences2023
Optimization of Sirius Red-Based Microplate Assay to Investigate Collagen Production In Vitro.
Article in International journal of molecular sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 18 citations in OpenAlex.
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- In-situ fabrication of a soluble PVP/ snail mucus-based fibrous facial mask via handheld electrospinning.Scientific reports · 2026Article
- Defining characteristics of mesenchymal stem cell-derived matrix-bound nanovesicles compared to conditioned culture medium extracellular vesicles.bioRxiv : the preprint server for biology · 2026Article
- Thermoresponsive Sol-Gel System Incorporating Oleuropein-Rich Olive Leaf Extract for Enhanced Wound Healing and Antibiofilm Activity.Gels (Basel, Switzerland) · 2026Article
- Self-assembling peptide nanofibers as growth factor-mimicking scaffolds enhancing the bone regeneration potential of nanoceramics: a triad ofRSC advances · 2025Article
- The First Simplified Heparan Sulphate-Alginate Hybrid Trisaccharides: Synthesis and Biological Effects on Human Dermal Fibroblasts.International journal of molecular sciences · 2025Article
- Effects of a single application of hydrolyzed fish collagen on dermal protein expression and tissue architecture in human skin models.Scientific reports · 2025Article
- The Effect of Rosavin, a Characteristic Compound ofInternational journal of molecular sciences · 2025Article
- Lamellarin D Acts as an Inhibitor of Type I Collagen Production.ChemMedChem · 2025Article
- Liver-targeted degradation of BRD4 reverses hepatic fibrosis and enhances metabolism in murine models.Theranostics · 2025Article
- Liposome Encapsulation of the Palmitoyl-KTTKS Peptide: Structural and Functional Characterization.Pharmaceutics · 2024Article
- Anti-astmatic effect of ROCK inhibitor, GSK429286 A, in experimentally induced allergic airway inflammation.International journal of immunopathology and pharmacologyArticle
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Tissue fibrosis is characterized by chronic fibroblast activation and consequently excessive accumulation of collagen-rich extracellular matrix. In vitro microplate-based assays are essential to investigate the underlying mechanism and the effect of antifibrotic drugs. In this study, in the absence of a gold-standard method, we optimized a simple, cost-effective, Sirius Red-based colorimetric measurement to determine the collagen production of fibroblasts grown on 96-well tissue culture plates. Based on our findings, the use of a serum-free medium is recommended to avoid aspecific signals, while ascorbate supplementation increases the collagen production of fibroblasts. The cell-associated collagens can be quantified by Sirius Red staining in acidic conditions followed by alkaline elution. Immature collagens can be precipitated from the culture medium by acidic Sirius Red solution, and after subsequent centrifugation and washing steps, their amount can be also measured. Increased attention has been paid to optimizing the assay procedure, including incubation time, temperature, and solution concentrations. The resulting assay shows high linearity and sensitivity and could serve as a useful tool in fibrosis-related basic research as well as in preclinical drug screening.
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