ArticleInternational journal of molecular sciences2023
A Comparative Study of Different Protocols for Isolation of Murine Neutrophils from Bone Marrow and Spleen.
Article in International journal of molecular sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 13 citations in OpenAlex.
- Neutrophils in cancer.Nature reviews. Cancer · 2026Review
- Iridium complex-loaded biomimetic vesicles enable enhanced photodynamic therapy and immune modulation.Microsystems & nanoengineering · 2026Article
- Cyclophosphamide-Mediated Induction of Myeloid-Derived Suppressor Cells In Vivo: Kinetics of Accumulation, Immune Profile, and Immunomodulation by Oleanane-Type Triterpenoids.International journal of molecular sciences · 2026Article
- A Comparison of Neutrophil Secretion of Cytokines and NET Formation in Response to Lipopolysaccharide and Group BJournal of inflammation research · 2026Article
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- Immunomodulatory potential of Toxoplasma gondii lysate in C57BL/6 mouse model of autoimmune encephalomyelitis.AMB Express · 2025Article
- Pulmozyme Ameliorates LPS-Induced Lung Fibrosis but Provokes Residual Inflammation by Modulating Cell-Free DNA Composition and Controlling Neutrophil Phenotype.Biomolecules · 2025Article
- Regulation of neutrophil extracellular trap formation by c-di-GMP inFrontiers in immunology · 2025Article
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- Two transcriptionally and functionally distinct waves of neutrophils during mouse acute liver injury.Hepatology communications · 2024Article
- Tumor- and Fibroblast-Derived Cell-Free DNAs Differently Affect the Progression of B16 Melanoma In Vitro and In Vivo.International journal of molecular sciences · 2024Article
- Nanoparticles containing intracellular proteins modulate neutrophil functional and phenotypic heterogeneity.Frontiers in immunology · 2024Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Neutrophils are considered as the main player in innate immunity. In the last few years, it has been shown that they are involved in different physiological conditions and diseases. However, progress in the field of neutrophil biology is relatively slow due to existing difficulties in neutrophil isolation and maintenance in culture. Here we compare four protocols based on density-gradient and immunomagnetic methods for isolation of murine neutrophils from bone marrow and spleen. Neutrophil isolation was performed using Ficoll 1.077/1.119 g/mL density gradient, Ficoll 1.083/1.090/1.110 g/mL density gradient and immunomagnetic method of negative and positive selection. The different protocols were compared with respect to sample purity, cell viability, yield, and cost. The functionality of isolated neutrophils was checked by NETosis analysis and neutrophil oxidative burst test. Obtained data revealed that given purity/yield/viability/cost ratio the protocol based on cell centrifugation on Ficoll 1.077/1.119 g/mL density gradient is recommended for isolation of neutrophils from bone marrow, whereas immunomagnetic method of positive selection using Dynabeads is recommended for isolation of splenic neutrophils.
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Registered trials
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