ArticleACS applied materials & interfaces2024
Region-Directed Enzyme Immobilization through Engineering Protein Surface with Histidine Clusters.
Article in ACS applied materials & interfaces, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 27 citations in OpenAlex.
- Eutectozymes as Soft Hybrid Materials for Advanced Biocatalysis.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- MartiniSurf: Automated Simulations of Surface-Immobilized Biomolecular Systems with Martini.Journal of chemical information and modeling · 2026Article
- Directed Immobilization of Horseradish Peroxidase Using a Covalently Attached Competitive Inhibitor.Molecules (Basel, Switzerland) · 2026Article
- How to Enhance the Applicability of Metal Ion Affinity-bound Biocatalysts? Comprehensive Overview and Case Study With a Phenylalanine Ammonia Lyase and an Amine Transaminase.Chembiochem : a European journal of chemical biology · 2026Article
- Mechanistic Determinants of Oriented Enzyme Immobilization from Martini Simulations.The journal of physical chemistry letters · 2026Article
- The Sabatier principle governs the performance of self-sufficient heterogeneous biocatalysts for redox biotransformations.Cell reports. Physical science · 2025Article
- Immobilization of Thrombin on Agarose-Based Supports for Affinity Tag Removal.Biomacromolecules · 2025Article
- An Artificial Metal-Free Peroxidase Designed Using a Ferritin Cage for Bioinspired Catalysis.Angewandte Chemie (International ed. in English) · 2025Article
- Advances and Challenges in the Development of Immobilized Enzymes for Batch and Flow Biocatalyzed Processes.ChemSusChem · 2025Review
- A Comprehensive Guide to Enzyme Immobilization: All You Need to Know.Molecules (Basel, Switzerland) · 2025Review
- Loop engineering of enzymes to control their immobilization and ultimately fabricate more efficient heterogeneous biocatalysts.Protein science : a publication of the Protein Society · 2025Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enzyme immobilization is a key enabling technology for a myriad of industrial applications, yet immobilization science is still too empirical to reach highly active and robust heterogeneous biocatalysts through a general approach. Conventional protein immobilization methods lack control over how enzymes are oriented on solid carriers, resulting in negative conformational changes that drive enzyme deactivation. Site-selective enzyme immobilization through peptide tags and protein domains addresses the orientation issue, but this approach limits the possible orientations to the N- and C-termini of the target enzyme. In this work, we engineer the surface of two model dehydrogenases to introduce histidine clusters into flexible regions not involved in catalysis, through which immobilization is driven. By varying the position and the histidine density of the clusters, we create a small library of enzyme variants to be immobilized on different carriers functionalized with different densities of various metal chelates (Co
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