Evidence map›Paper›PMID 38134472›Full record

ArticleBiomaterials2024

Custom-engineered hydrogels for delivery of human iPSC-derived neurons into the injured cervical spinal cord.

V M Doulames, L M Marquardt, M E Hefferon, N J Baugh, R A Suhar, A T Wang, K R Dubbin, J M Weimann, T D Palmer, G W Plant and 1 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed, 1 pooled it
7.9field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 1 synthesis or guideline pooled it, 25 citations in OpenAlex.

  1. Pooled it
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  15. Engineered Protein Hydrogels as Biomimetic Cellular Scaffolds.Advanced materials (Deerfield Beach, Fla.) · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 1 institution in 1 country.

V M DoulamesDepartment of Materials Science and Engineering, Stanford University, Stanford, CA, 94305, USA.
L M MarquardtDepartment of Materials Science and Engineering, Stanford University, Stanford, CA, 94305, USA.
M E HefferonDepartment of Materials Science and Engineering, Stanford University, Stanford, CA, 94305, USA.
N J BaughDepartment of Materials Science and Engineering, Stanford University, Stanford, CA, 94305, USA.
R A SuharDepartment of Materials Science and Engineering, Stanford University, Stanford, CA, 94305, USA.
A T WangDepartment of Materials Science and Engineering, Stanford University, Stanford, CA, 94305, USA.
K R DubbinDepartment of Materials Science and Engineering, Stanford University, Stanford, CA, 94305, USA.
J M WeimannDepartment of Neurosurgery, Stanford University School of Medicine, Stanford, CA, 94305, USA.
T D PalmerDepartment of Neurosurgery, Stanford University School of Medicine, Stanford, CA, 94305, USA.
G W PlantDepartment of Neurosurgery, Stanford University School of Medicine, Stanford, CA, 94305, USA. Electronic address: giles.plant@osumc.edu.
S C HeilshornDepartment of Materials Science and Engineering, Stanford University, Stanford, CA, 94305, USA. Electronic address: heilshorn@stanford.edu.
Stanford University · US

Funding

Injectable Hydrogels to Protect Transplanted Cells from HypoxiaR01EB027666 · NIBIB · STANFORD UNIVERSITY · PI HEILSHORN, SARAH C, PLANT, GILES · 2019 to 2022
$1.4M
Engineered biomaterials to modulate cell-cell signaling for the robust expansion of stem cellsR01EB027171 · NIBIB · STANFORD UNIVERSITY · PI HEILSHORN, SARAH C · 2019 to 2022
$1.4M
NIBIB NIH HHS R01 EB027171NIBIB NIH HHS R01 EB027666
6 · The paper itself

Abstract

Cervical damage is the most prevalent type of spinal cord injury clinically, although few preclinical research studies focus on this anatomical region of injury. Here we present a combinatorial therapy composed of a custom-engineered, injectable hydrogel and human induced pluripotent stem cell (iPSC)-derived deep cortical neurons. The biomimetic hydrogel has a modular design that includes a protein-engineered component to allow customization of the cell-adhesive peptide sequence and a synthetic polymer component to allow customization of the gel mechanical properties. In vitro studies with encapsulated iPSC-neurons were used to select a bespoke hydrogel formulation that maintains cell viability and promotes neurite extension. Following injection into the injured cervical spinal cord in a rat contusion model, the hydrogel biodegraded over six weeks without causing any adverse reaction. Compared to cell delivery using saline, the hydrogel significantly improved the reproducibility of cell transplantation and integration into the host tissue. Across three metrics of animal behavior, this combinatorial therapy significantly improved sensorimotor function by six weeks post transplantation. Taken together, these findings demonstrate that design of a combinatorial therapy that includes a gel customized for a specific fate-restricted cell type can induce regeneration in the injured cervical spinal cord.

Indexed as

Cervical CordInduced Pluripotent Stem CellsSpinal Cord InjuriesAnimalsHumansHydrogelsNeuronsRatsReproducibility of ResultsSpinal CordHydrogelsBiomaterialsCell transplantationHydrogelInduced pluripotent stem cellSpinal cord injury

Identifiers

PMID38134472
PMCPMC10846596
OpenAlexW4388749604

What OpenQuestion holds

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LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.