Evidence map›Paper›PMID 38133393›Full record

ArticleToxics2023

Proteomic Analysis of the Mitochondrial Responses in P19 Embryonic Stem Cells Exposed to Florfenicol.

Zhihua Dong, Xueke Hou, Xueying Wang, Zihui Shen, Huiqing Pang, Lingli Chen, Zhihong Yin, Fei Ren, Weiguo Li, Yaming Ge and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Toxics, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
1.1field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 3 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 1 country.

Zhihua DongCollege of Animal Science and Technology, Henan Institute of Science and Technology, Xinxiang 453003, China.
Xueke HouCollege of Animal Science and Technology, Henan Institute of Science and Technology, Xinxiang 453003, China.
Xueying WangCollege of Animal Science and Technology, Henan Institute of Science and Technology, Xinxiang 453003, China.
Zihui ShenCollege of Animal Science and Technology, Henan Institute of Science and Technology, Xinxiang 453003, China.
Huiqing PangCollege of Animal Science and Technology, Henan Institute of Science and Technology, Xinxiang 453003, China.
Lingli ChenCollege of Animal Science and Technology, Henan Institute of Science and Technology, Xinxiang 453003, China.
Zhihong YinCollege of Animal Science and Technology, Henan Institute of Science and Technology, Xinxiang 453003, China.
Fei RenCollege of Animal Science and Technology, Henan Institute of Science and Technology, Xinxiang 453003, China.
Weiguo LiPostdoctoral Research Station in Biological Sciences, Henan Normal University, Xinxiang 453003, China.
Yaming GeCollege of Animal Science and Technology, Henan Institute of Science and Technology, Xinxiang 453003, China.
Hongmei NingCollege of Animal Science and Technology, Henan Institute of Science and Technology, Xinxiang 453003, China.
Dongfang HuCollege of Animal Science and Technology, Henan Institute of Science and Technology, Xinxiang 453003, China.
Henan Institute of Science and Technology · CNHenan Normal University · CN

Funding

the Chinese Postdoctoral Science Foundation 2019M652548the Key Scientific Research Projects of Universities in Henan province 23A230013the National Natural Science Foundation of China 32002352the Scientific and Technological Foundation of Henan Province 222102110430, 212102110102, 222102310027
6 · The paper itself

Abstract

Florfenicol (FLO) has been shown to elicit diverse toxic effects in plants, insects, and mammals. Previously, our investigations revealed that FLO induced abnormal cardiac development and early embryonic mortality in chicken embryos. However, the effect of FLO on mitochondrial responses in stem cells remains unclear. In this study, we show that FLO significantly diminishes proliferation viability and obstructs the directed differentiation of P19 stem cells (P19SCs) into cardiomyocytes. Proteomic analysis revealed 148 differentially expressed proteins in response to FLO. Functional analysis has pinpointed FLO interference with biological processes associated with oxidative phosphorylation within the mitochondria. In alignment with the results of proteomic analysis, we confirmed that FLO inhibits the expression of both nuclear DNA-encoded and mitochondrial DNA-encoded subunits of the electron transport chain. Subsequent experiments demonstrated that FLO disrupts mitochondrial dynamics and induces the mitochondrial unfolded protein response to maintain mitochondrial homeostasis. These findings collectively highlight the significance of mitochondrial dynamics and the mitochondrial unfolded protein response to mediate the decreased proliferation viability and directed differentiation potential in P19SCs treated with FLO. In conclusion, this study provides a comprehensive overview of mitochondrial responses to FLO-induced cytotoxicity and enhances our understandings of the molecular mechanisms underlying FLO-induced embryonic toxicity.

Indexed as

embryonic toxicityflorfenicolmitochondrial dynamicsproteomic analysis

Identifiers

PMID38133393
PMCPMC10747307
OpenAlexW4389385674

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.