Evidence map›Paper›PMID 38132771›Full record

ArticleJournal of fungi (Basel, Switzerland)2023

An Efficient CRISPR/Cas9 Genome Editing System for a

Yi Tan, Xianglin Yu, Zhigang Zhang, Jialin Tian, Na Feng, Chuanhong Tang, Gen Zou, Jingsong Zhang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of fungi (Basel, Switzerland), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
2.9field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 12 citations in OpenAlex.

  1. Evaluation ofFoods (Basel, Switzerland) · 2026
    Article
  2. Article
  3. Development of a CRISPR/Cas9 Genome Editing System in DikaryoticJournal of fungi (Basel, Switzerland) · 2026
    Article
  4. Integrative Genomic and Transcriptomic Analysis of White-Rot FungiJournal of fungi (Basel, Switzerland) · 2026
    Article
  5. Screening of Monokaryotic Strains ofJournal of fungi (Basel, Switzerland) · 2025
    Article
  6. Unraveling bioactive potential and production inChinese herbal medicines · 2025
    Review
  7. Review
  8. Gene Editing inJournal of fungi (Basel, Switzerland) · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Yi TanNational Engineering Research Center of Edible Fungi, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
Xianglin YuCollege of Food Sciences & Technology, Shanghai Ocean University, Shanghai 201306, China.
Zhigang ZhangCollege of Life Sciences, Shanghai Normal University, Shanghai 200234, China.
Jialin TianCollege of Food Sciences & Technology, Shanghai Ocean University, Shanghai 201306, China.
Na FengNational Engineering Research Center of Edible Fungi, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
Chuanhong TangNational Engineering Research Center of Edible Fungi, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
Gen ZouNational Engineering Research Center of Edible Fungi, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.ORCID 0000-0001-5574-1824
Jingsong ZhangNational Engineering Research Center of Edible Fungi, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
Shanghai Academy of Agricultural Sciences · CNShanghai Ocean University · CNShanghai Normal University · CN

Funding

Wellcome Trust 202309
6 · The paper itself

Abstract

The CRISPR/Cas9 system has become a popular approach to genome editing. Compared with the plasmid-dependent CRISPR system, the ribonucleoprotein (RNP) complex formed by the in vitro assembly of Cas9 and single-guide RNA (sgRNA) has many advantages. However, only a few examples have been reported and the editing efficiency has been relatively low. In this study, we developed and optimized an RNP-mediated CRISPR/Cas9 genome editing system for the monokaryotic strain L1 from the

Indexed as

CRISPR/Cas9Ganoderma lucidumgenome editingribonucleoproteins (RNPs)the orotidine 5′-monophosphate decarboxylase gene (ura3)

Identifiers

PMID38132771
PMCPMC10745038
OpenAlexW4389335463

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.