Evidence map›Paper›PMID 38132172›Full record

ArticleCells2023

Enhanced Osteocyte Differentiation: Cathepsin D and L Secretion by Human Adipose-Derived Mesenchymal Stem Cells.

Jung-Won Choi, Soyeon Lim, Seung Eun Jung, Seongtae Jeong, Hanbyeol Moon, Byeong-Wook Song, Il-Kwon Kim, Seahyoung Lee, Ki-Chul Hwang, Sang Woo Kim

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cells, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.4field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Jung-Won ChoiMedical Science Research Institute, College of Medicine, Catholic Kwandong University, Incheon Metropolitan City 22711, Republic of Korea.
Soyeon LimDepartment of Convergence Science, College of Medicine, Catholic Kwandong University, Incheon Metropolitan City 22711, Republic of Korea.ORCID 0000-0001-9814-0682
Seung Eun JungMedical Science Research Institute, College of Medicine, Catholic Kwandong University, Incheon Metropolitan City 22711, Republic of Korea.ORCID 0000-0002-5143-703X
Seongtae JeongThe Interdisciplinary Graduate Program in Integrative Biotechnology, Yonsei University, Seoul 03722, Republic of Korea.ORCID 0000-0002-9705-531X
Hanbyeol MoonDepartment of Integrated Omics for Biomedical Sciences, Graduate School, Yonsei University, Seoul 03722, Republic of Korea.
Byeong-Wook SongDepartment of Convergence Science, College of Medicine, Catholic Kwandong University, Incheon Metropolitan City 22711, Republic of Korea.ORCID 0000-0002-9962-0941
Il-Kwon KimDepartment of Convergence Science, College of Medicine, Catholic Kwandong University, Incheon Metropolitan City 22711, Republic of Korea.
Seahyoung LeeDepartment of Convergence Science, College of Medicine, Catholic Kwandong University, Incheon Metropolitan City 22711, Republic of Korea.
Ki-Chul HwangDepartment of Convergence Science, College of Medicine, Catholic Kwandong University, Incheon Metropolitan City 22711, Republic of Korea.
Sang Woo KimDepartment of Convergence Science, College of Medicine, Catholic Kwandong University, Incheon Metropolitan City 22711, Republic of Korea.ORCID 0000-0002-3144-4822
Catholic Kwandong University · KRYonsei University · KR

Funding

National Research Foundation of Korea NRF-2020R1I1A2073643National Research Foundation of Korea NRF-2021R1A2C1005280
6 · The paper itself

Abstract

Adipose-derived mesenchymal stem cells (ASCs) have the potential to differentiate into bone, cartilage, fat, and neural cells and promote tissue regeneration and healing. It is known that they can have variable responses to hypoxic conditions. In the present study, we aimed to explore diverse changes in the cells and secretome of ASCs under a hypoxic environment over time and to present the possibility of ASCs as therapeutic agents from a different perspective. The expression differences of proteins between normoxic and hypoxic conditions (6, 12, or 24 h) were specifically investigated in human ASCs using 2-DE combined with MALDI-TOF MS analysis, and secreted proteins in ASC-derived conditioned media (ASC-derived CM) were examined by an adipokine array. In addition, genetic and/or proteomic interactions were assessed using a DAVID and miRNet functional annotation bioinformatics analysis. We found that 64 and 5 proteins were differentially expressed in hypoxic ASCs and in hypoxic ASC-derived CM, respectively. Moreover, 7 proteins among the 64 markedly changed spots in hypoxic ASCs were associated with bone-related diseases. We found that two proteins, cathepsin D (CTSD) and cathepsin L (CTSL), identified through an adipokine array independently exhibited significant efficacy in promoting osteocyte differentiation in bone-marrow-derived mesenchymal stem cells (BM-MSCs). This finding introduces a promising avenue for utilizing hypoxia-preconditioned ASC-derived CM as a potential therapeutic approach for bone-related diseases.

Indexed as

Adipose TissueMesenchymal Stem CellsAdipokinesCathepsin DHumansHypoxiaOsteocytesProteomicsAdipokinesCathepsin Dadipose-derived mesenchymal stem cellshypoxic conditionosteoblast differentiationsecretometwo-dimensional electrophoresis

Identifiers

PMID38132172
PMCPMC10742070
OpenAlexW4389925447

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.