Evidence map›Paper›PMID 38131003›Full record

ArticleAdvanced functional materials2023

A Facile Strategy for the Fabrication of Cell-laden Porous Alginate Hydrogels Based on Two-phase Aqueous Emulsions.

Wen Xue, Donghee Lee, Yunfan Kong, Mitchell Kuss, Ying Huang, Taesung Kim, Soonkyu Chung, Andrew T Dudley, Seung-Hyun Ro, Bin Duan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced functional materials, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Article
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  8. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Wen XueCollege of Biological Science and Medical Engineering, Donghua University, Shanghai 201620, China.; Department of Internal Medicine, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA.; Mary & Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA.
Donghee LeeMary & Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA.; Department of Surgery, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA.
Yunfan KongDepartment of Internal Medicine, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA.; Mary & Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA.; Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA.
Mitchell KussDepartment of Internal Medicine, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA.; Mary & Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA.
Ying HuangEppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA.
Taesung KimDepartment of Biochemistry and the Redox Biology Center, University of Nebraska-Lincoln, Lincoln, Nebraska 68588, USA.
Soonkyu ChungDepartment of Nutrition, University of Massachusetts Amherst, Amherst, Massachusetts 01003, USA.
Andrew T DudleyMary & Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA.; Department of Genetics, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA.
Seung-Hyun RoDepartment of Biochemistry and the Redox Biology Center, University of Nebraska-Lincoln, Lincoln, Nebraska 68588, USA.ORCID 0000-00002-7744-7615
Bin DuanDepartment of Internal Medicine, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA.; Mary & Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA.; Department of Surgery, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA.; Department of Mechanical and Materials Engineering, University of Nebraska-Lincoln, Lincoln, Nebraska 68588, USA.ORCID 0000-0002-5647-3793

Funding

The role of stress in the fetal origin of obesity and metabolic dysfunctionP20GM104320 · NIGMS · UNIVERSITY OF NEBRASKA LINCOLN · PI ZEMPLENI, JANOS · 2014 to 2024
$24.9M
A Hydrogel Ionic Circuit-Based Electrical Stimulation System for Restoration of Denervated Muscles After Peripheral Nerve InjuriesR21AR078439 · NIAMS · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI DUAN, BIN, ZHAO, SIWEI · 2021 to 2022
$378k
NIAMS NIH HHS R21 AR078439NIGMS NIH HHS P20 GM104320
6 · The paper itself

Abstract

Porous alginate hydrogels possess many advantages as cell carriers. However, current pore generation methods require either complex or harsh fabrication processes, toxic components, or extra purification steps, limiting the feasibility and affecting the cellular survival and function. In this study, a simple and cell-friendly approach to generate highly porous cell-laden alginate hydrogels based on two-phase aqueous emulsions is reported. The pre-gel solutions, which contain two immiscible aqueous phases of alginate and caseinate, are crosslinked by calcium ions. The porous structure of the hydrogel construct is formed by subsequently removing the caseinate phase from the ion-crosslinked alginate hydrogel. Those porous alginate hydrogels possess heterogeneous pores around 100 μm and interconnected paths. Human white adipose progenitors (WAPs) encapsulated in these hydrogels self-organize into spheroids and show enhanced viability, proliferation, and adipogenic differentiation, compared to non-porous constructs. As a proof of concept, this porous alginate hydrogel platform is employed to prepare core-shell spheres for coculture of WAPs and colon cancer cells, with WAP clusters distributed around cancer cell aggregates, to investigate cellular crosstalk. This efficacious approach is believed to provide a robust and versatile platform for engineering porous-structured alginate hydrogels for applications as cell carriers and in disease modeling.

Indexed as

adipose-cancer interactioncore-shellporous hydrogelspheroidtwo-phase aqueous emulsions

Identifiers

PMID38131003
PMCPMC10732541

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.