ArticleNature methods2024
The Cousa objective: a long-working distance air objective for multiphoton imaging in vivo.
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Article in Nature methods, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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37 citing papers in PubMed.
- Methods, Trade-offs, and Opportunities in High-speed Optical Microscopy for Neural Voltage imaging.Nature photonics · 2026Article
- Large-FOV, dual-region, two-photon fluorescence lifetime imaging microscopy (LD-2P-FLIM) for multiparameter and quantitative brain function imaging.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Drug self-administration in head-fixed mice.Nature protocols · 2026Review
- Evidence that interglomerular inhibition generates non-monotonic concentration-response relationships in mitral/tufted glomeruli in the mouse olfactory bulb.The Journal of physiology · 2026Article
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- Ultra-wide-field, deep, adaptive two-photon microscopy for multi-scale neuronal imaging.Light, science & applications · 2026Article
- Intravital mesoscale optical imaging: challenges, techniques, and future perspectives.Biophysics reports · 2026Article
- Cortex-Wide Cellular Imaging in Freely Locomoting Mice Using Cortex Camera Array Microscope (CortexCAM).bioRxiv : the preprint server for biology · 2026Article
- Multi-scale imaging and recording of in-vivo neural activity.BMB reports · 2026Review
- Memory erasure by dopamine-gated retrospective learning.bioRxiv : the preprint server for biology · 2026Article
- CaBLAM: a high-contrast bioluminescent CaNature methods · 2026Article
- Simultaneous thermal and large field-of-view two-photon imaging enables optimization of signal-to-noise.Neurophotonics · 2026Article
- Fully 3D-printed endomicroscopic objective for two-photon, multi-wavelength excitation microscopy.Biomedical optics express · 2026Article
- Open-source modular field-programmable gate array system for two-photon mesoscope enabling multiarea, multidepth neural activity recording and lifetime imaging.Neurophotonics · 2026Article
- Modulating Chromophore Flexibility in GEVIs through Threonine-Based Molecular Switches Reveals an Influence of Membrane Curvature on Protein Activity.ACS sensors · 2025Article
- A cortex-wide multimodal microscope for simultaneous CaNature communications · 2025Article
- Three-photon microscopy: an emerging technique for deep intravital brain imaging.Nature reviews. Neuroscience · 2025Review
- Evidence that interglomerular inhibition generates non-monotonic concentration-response relationships in mitral/tufted glomeruli in the mouse olfactory bulb.bioRxiv : the preprint server for biology · 2025Article
- CaBLAM! A high-contrast bioluminescent CabioRxiv : the preprint server for biology · 2025Article
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Authors and funding
28 authors.
Che-Hang YuDepartment of Electrical and Computer Engineering, University of California Santa Barbara, Santa Barbara, CA, USA. chehangyu@ucsb.edu.ORCID http://orcid.org/0000-0002-0353-9752
Yiyi YuDepartment of Electrical and Computer Engineering, University of California Santa Barbara, Santa Barbara, CA, USA.ORCID http://orcid.org/0000-0003-1053-413X
Liam M AdsitDepartment of Molecular, Cellular, and Developmental Biology, University of California Santa Barbara, Santa Barbara, CA, USA.
Jeremy T ChangMax Planck Florida Institute for Neuroscience, Jupiter, FL, USA.ORCID http://orcid.org/0000-0002-8802-5338
Jad BarchiniMax Planck Florida Institute for Neuroscience, Jupiter, FL, USA.
Andrew H MoberlyDepartment of Neuroscience, Yale University, New Haven, CT, USA.
Hadas BenistyDepartment of Neuroscience, Yale University, New Haven, CT, USA.
Jinkyung KimDepartment of Otolaryngology, Washington University School of Medicine, St. Louis, MO, USA.
Brent K YoungSpencer Center for Vision Research, Byers Eye Institute, School of Medicine, Stanford University, Palo Alto, CA, USA.ORCID http://orcid.org/0000-0002-4185-0277
Kathleen HengSpencer Center for Vision Research, Byers Eye Institute, School of Medicine, Stanford University, Palo Alto, CA, USA.
Deano M FarinellaDepartment of Neuroscience, University of Minnesota, Minneapolis, MN, USA.
Austin LeikvollDepartment of Neuroscience, University of Minnesota, Minneapolis, MN, USA.ORCID http://orcid.org/0000-0003-3761-9475
Rishaab PavanDepartment of Biomedical Engineering, University of Minnesota, Minneapolis, MN, USA.
Rachel VisteinDepartment of Molecular and Comparative Pathobiology, and Zanvyl Krieger Mind/Brain Institute, Johns Hopkins University, Baltimore, MD, USA.ORCID http://orcid.org/0000-0002-2172-9947
Brandon R NanfitoSolomon H. Snyder Department of Neuroscience, and Zanvyl Krieger Mind/Brain Institute, Johns Hopkins University, Baltimore, MD, USA.
David G C HildebrandLaboratory of Neural Systems, The Rockefeller University, New York, NY, USA.ORCID http://orcid.org/0000-0002-2079-3517
Santiago Otero-CoronelLaboratory of Neural Systems, The Rockefeller University, New York, NY, USA.ORCID http://orcid.org/0000-0003-4633-7111
Alipasha VaziriLaboratory of Neurotechnology and Biophysics, The Rockefeller University, New York, NY, USA.ORCID http://orcid.org/0000-0001-8346-7551
Jeffrey L GoldbergSpencer Center for Vision Research, Byers Eye Institute, School of Medicine, Stanford University, Palo Alto, CA, USA.ORCID http://orcid.org/0000-0002-1390-7360
Anthony J RicciDepartment of Otolaryngology, Stanford University School of Medicine, Stanford University, Stanford, CA, USA.ORCID http://orcid.org/0000-0002-1706-8904
David FitzpatrickMax Planck Florida Institute for Neuroscience, Jupiter, FL, USA.
Jessica A CardinDepartment of Neuroscience, Yale University, New Haven, CT, USA.ORCID http://orcid.org/0000-0002-8209-5466
Michael J HigleyDepartment of Neuroscience, Yale University, New Haven, CT, USA.ORCID http://orcid.org/0000-0002-5424-6730
Gordon B SmithDepartment of Neuroscience, University of Minnesota, Minneapolis, MN, USA.ORCID http://orcid.org/0000-0002-1107-3884
Prakash KaraDepartment of Neuroscience, University of Minnesota, Minneapolis, MN, USA.
Kristina J NielsenSolomon H. Snyder Department of Neuroscience, and Zanvyl Krieger Mind/Brain Institute, Johns Hopkins University, Baltimore, MD, USA.ORCID http://orcid.org/0000-0002-9155-2972
Ikuko T SmithDepartment of Molecular, Cellular, and Developmental Biology, University of California Santa Barbara, Santa Barbara, CA, USA.
Spencer LaVere SmithDepartment of Electrical and Computer Engineering, University of California Santa Barbara, Santa Barbara, CA, USA. sls@ucsb.edu.ORCID http://orcid.org/0000-0002-2021-7034
Funding
Tonotopic variations in mechano-electric transductionR01DC003896 · NIDCD · STANFORD UNIVERSITY · PI RICCI, ANTHONY J · 1999 to 2022
$11.1MThe Development of Direction Selectivity in Visual CortexR01EY011488 · NEI · MAX PLANCK FLORIDA CORPORATION · PI FITZPATRICK, DAVID · 1996 to 2025
$10.6MStanford Vision Research CoreP30EY026877 · NEI · STANFORD UNIVERSITY · PI TIRIN MOORE · 2017 to 2026
$8.0MFunctional Synaptic Architecture of Visual CortexR01EY006821 · NEI · MAX PLANCK FLORIDA CORPORATION · PI FITZPATRICK, DAVID · 1987 to 2020
$7.2MYale Core Grant for Vision ResearchP30EY026878 · NEI · YALE UNIVERSITY · PI In-Jung Kim · 2016 to 2026
$7.1MTraining Veterinarians for Careers in Biomedical ResearchT32OD011089 · OD · JOHNS HOPKINS UNIVERSITY · PI JOSEPH L MANKOWSKI · 2012 to 2026
$6.7MBridging cellular and systems neuroscience: synaptic dynamics underlying behaviorDP1EY033975 · NEI · YALE UNIVERSITY · PI HIGLEY, MICHAEL JAMES · 2021 to 2025
$5.9MCellular mechanisms of GABAergic inhibition in neocortical dendritesR01MH099045 · NIMH · YALE UNIVERSITY · PI Michael James Higley · 2012 to 2026
$5.7MOptimization, Application, and Dissemination of Imaging Modules for High-speed Mesoscopic Volumetric Recording of Neuroactivity in Scattering BrainsU01NS126057 · NINDS · ROCKEFELLER UNIVERSITY · PI VAZIRI, ALIPASHA · 2022 to 2025
$5.0MOptimization, application and dissemination of high-speed hybrid multiphoton volumetric imaging technologiesU01NS115530 · NINDS · ROCKEFELLER UNIVERSITY · PI LOSONCZY, ATTILA, VAZIRI, ALIPASHA · 2020 to 2023
$4.7MTraining Program in NeuroscienceT32NS091018 · NINDS · JOHNS HOPKINS UNIVERSITY · PI JAMES J KNIERIM, Daniel Hans O'Connor · 2015 to 2026
$4.1MInhibitory regulation of cortical visual processingR01EY022951 · NEI · YALE UNIVERSITY · PI CARDIN, JESSICA A · 2013 to 2025
$4.0MNEI NIH HHS DP1 EY033975NEI NIH HHS F32 EY031133NEI NIH HHS P30 EY026877NEI NIH HHS P30 EY026878NEI NIH HHS R01 EY006821NEI NIH HHS R01 EY011488NEI NIH HHS R01 EY022951NEI NIH HHS R01 EY024294NEI NIH HHS R01 EY030893NEI NIH HHS R01 EY032416NEI NIH HHS R01 EY035378NIDCD NIH HHS R01 DC003896NIDCD NIH HHS R21 DC020325NIH HHS T32 OD011089NIMH NIH HHS R01 MH099045NINDS NIH HHS R01 NS091335NINDS NIH HHS R01 NS121919NINDS NIH HHS R01 NS128079NINDS NIH HHS T32 NS091018NINDS NIH HHS U01 NS103488NINDS NIH HHS U01 NS115530NINDS NIH HHS U01 NS115585NINDS NIH HHS U01 NS126057
Abstract
Multiphoton microscopy can resolve fluorescent structures and dynamics deep in scattering tissue and has transformed neural imaging, but applying this technique in vivo can be limited by the mechanical and optical constraints of conventional objectives. Short working distance objectives can collide with compact surgical windows or other instrumentation and preclude imaging. Here we present an ultra-long working distance (20 mm) air objective called the Cousa objective. It is optimized for performance across multiphoton imaging wavelengths, offers a more than 4 mm
Indexed as
Coloring AgentsMicroscopy, Fluorescence, MultiphotonAnimalsColoring Agents
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