ArticleNature reviews. Methods primers2022
Two-photon calcium imaging of neuronal activity.
Article in Nature reviews. Methods primers, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
76 citing papers in PubMed.
- CLOK: a chemigenetic multicolor labeling system to visualize neuronal birthdate and circuit integration.Nature neuroscience · 2026Article
- A hybrid micro-ECoG for functionally targeted multi-site and multi-scale investigation.Cell reports methods · 2026Article
- Protocol for longitudinal two-photon calcium imaging and holographic optogenetic manipulation to investigate memory in mice.STAR protocols · 2026Article
- Fast penalized generalized estimating equations for large longitudinal functional datasets.Biometrics · 2026Article
- NeuroSeg-MF: robust neuron segmentation in two-photon CaBiomedical optics express · 2026Article
- Analytical model enabling the correction of absorption and scattering in dual-color ratiometric multiphoton microscopy of brain tissue.Neurophotonics · 2026Article
- Optimization of spatial and temporal sampling resolution for optophysiology in large-scale two-photon calcium imaging.Biomedical optics express · 2026Article
- Review
- Article
- A hybrid micro-ECoG for functionally targeted multi-site and multi-scale investigation.bioRxiv : the preprint server for biology · 2026Article
- In Vivo Imaging of Neuronal Activity Shifts in the Somatosensory Cortex After Morphine Tolerance in Mice.Addiction biology · 2026Article
- Deriving functional network topology frombioRxiv : the preprint server for biology · 2026Article
- Evolving reservoir computers reveal bidirectional coupling between predictive power and emergent dynamics.Patterns (New York, N.Y.) · 2026Article
- Super-resolution functional photoacoustic microscopy via label-free cell tracking.Light, science & applications · 2026Article
- Cocaine induced astrocytic activation was associated with its vasoconstricting effects independent of its neuronal effects in the prefrontal cortex.Scientific reports · 2026Article
- A dual-color miniature endoscope for calcium imaging in behaving mice.bioRxiv : the preprint server for biology · 2026Article
- Review
- Multi-scale imaging and recording of in-vivo neural activity.BMB reports · 2026Review
- Functional ultrasound imaging in preclinical brain stimulation: from multimodal readouts to closed-loop neuromodulation.Frontiers in neuroscience · 2026Review
- Noise Sources and Strategies for Signal Quality Improvement in Biological Imaging: A Review Focused on Calcium and Cell Membrane Voltage Imaging.Biosensors · 2026Review
16 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
In vivo two-photon calcium imaging (2PCI) is a technique used for recording neuronal activity in the intact brain. It is based on the principle that, when neurons fire action potentials, intracellular calcium levels rise, which can be detected using fluorescent molecules that bind to calcium. This Primer is designed for scientists who are considering embarking on experiments with 2PCI. We provide the reader with a background on the basic concepts behind calcium imaging and on the reasons why 2PCI is an increasingly powerful and versatile technique in neuroscience. The Primer explains the different steps involved in experiments with 2PCI, provides examples of what ideal preparations should look like and explains how data are analysed. We also discuss some of the current limitations of the technique, and the types of solutions to circumvent them. Finally, we conclude by anticipating what the future of 2PCI might look like, emphasizing some of the analysis pipelines that are being developed and international efforts for data sharing.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.