ArticleBMC genomics2023
Transcriptome analysis of hypothalamus and pituitary tissues reveals genetic mechanisms associated with high egg production rates in Changshun green-shell laying hens.
Article in BMC genomics, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 9 citations in OpenAlex.
- Integrated multi-tissue transcriptomic analysis identifies hub genes associated with egg production recovery after fasting-induced molting.Poultry science · 2026Article
- Analysis of key regulatory genes for egg-laying performance in inner Mongolia bian chicken and prairie green chicken.Poultry science · 2026Article
- Dynamic synergistic interplay between ovarian antioxidant defense and angiogenesis sustains high egg production in laying hens.Journal of animal science and biotechnology · 2026Article
- Differential Expression of Hypothalamic Genes in Laying Hens Housed in Caged and Cage-Free Systems Under Commercial Conditions in the Tropics.Animals : an open access journal from MDPI · 2026Article
- Integrative multi-omics analysis reveals key regulatory nodes of abdominal fat deposition in broiler chickens.Poultry science · 2025Article
- Transcriptome profiling of hypothalamus-pituitary-ovary axis provides insights into egg-laying interval differences between european meat pigeons and shiqi pigeons.Frontiers in genetics · 2025Article
- Transcriptome Analysis of Hypothalamic-Pituitary-Ovarian Axis Reveals circRNAs Related to Egg Production of Bian Chicken.Animals : an open access journal from MDPI · 2024Article
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8 authors at 1 institution in 1 country.
Funding
Abstract
backgroundChangshun green-shell laying hens are unique to the Guizhou Province, China, and have high egg quality but relatively low yield. Egg production traits are regulated by the hypothalamus-pituitary-ovary axis. However, the underlying mechanism remains unclear. Thus, we conducted RNA sequencing of hypothalamic and pituitary tissues from low- and high-yielding Changshun green-shell laying hens to identify critical pathways and candidate genes involved in controlling the egg production rate.
resultsMore than 39 million clean reads per sample were obtained, and more than 82% were mapped to the Gallus gallus genome. Further analysis identified 1,817 and 1,171 differentially expressed genes (DEGs) in the hypothalamus and pituitary, respectively. Nineteen DEGs were upregulated in both the hypothalamus and pituitary of high-yielding chickens. The functions of these DEGs were mainly associated with ion transport or signal transduction. Gene set enrichment analysis revealed that the pathways enriched in the hypothalamus were mainly associated with gonadotropin-releasing hormone (GnRH) secretion, neurotransmitter release, and circadian rhythms. The pathways enriched in the pituitary were mainly associated with GnRH secretion, energy metabolism, and signal transduction. Five and four DEGs in the hypothalamus and pituitary, respectively, were selected randomly for qRT-PCR analysis. The expression trends determined via qRT-PCR were consistent with the RNA-seq results.
conclusionsThe current study identified 19 DEGs upregulated in both the hypothalamus and pituitary gland, which could provide an important reference for further studies on the molecular mechanisms underlying egg production in Changshun green-shell laying hens. In addition, enrichment analysis showed that GnRH secretion and signal transduction, especially neurotransmitter release, play crucial roles in the regulation of egg production.
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