ArticleNature communications2023
Reconfiguration of the reductive TCA cycle enables high-level succinic acid production by Yarrowia lipolytica.
Article in Nature communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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31 citing papers in PubMed, 74 citations in OpenAlex.
- Engineering nonconventional yeasts to produce carboxylic acids: Advances and Prospects.Synthetic and systems biotechnology · 2027Review
- Recent progress in microbial succinic acid production: integrating genome engineering and fermentation strategies.World journal of microbiology & biotechnology · 2026Review
- ExploringApplied and environmental microbiology · 2026Article
- Beyond petrochemicals: challenges and opportunities in industrial-scale biomanufacturing.Nature communications · 2026Review
- Coupled succinic acid fermentation and biogas upgrading enabled by in situ product recovery.Biotechnology for biofuels and bioproducts · 2026Article
- Perspectives for Advancing Biotechnological Succinic Acid Production.Microbial biotechnology · 2026Review
- Biotechnological applications of D-xylose-fermenting autochthonous yeasts in lignocellulosic biorefineries.Biotechnology for biofuels and bioproducts · 2026Review
- EngineeringSynthetic and systems biotechnology · 2026Article
- Combining multiplex metabolic engineering with adaptive evolution strategies for high-level succinic acid production inSynthetic and systems biotechnology · 2026Article
- Photoelectrocatalytic-microbial biohybrid for succinic acid synthesis.Nature communications · 2026Article
- Enhancing succinic acid production from lignocellulosic hydrolysates via reductive TCA pathway engineering in Klebsiella oxytoca.Bioprocess and biosystems engineering · 2026Article
- Selective utilization of formic acid and acetic acid in succinic acid fermentation broth to produce single-cell protein using Rhodotorula glutinis.Bioprocess and biosystems engineering · 2026Article
- Improved de novo production of prostaglandin FCommunications biology · 2026Article
- Succinic Acid Production by Engineered Mannheimia succiniciproducens and Its Use in Chemoenzymatic Poly(Butylene Succinate) Synthesis.Biotechnology and bioengineering · 2025Article
- Improving succinic acid production by tuning polysulfides metabolism in Yarrowia lipolytica.Microbial cell factories · 2025Article
- Xylose Metabolism Perturbation in Yarrowia lipolytica for Efficient Succinic Acid Bioproduction from Lignocellulosic Biomass.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Production of dicarboxylates from ω-amino acids using a cofactor- and co-substrate-freeEngineering microbiology · 2025Article
- The roles of NHEJ and TLS pathways in genomic alterations and phenotypic evolution in the yeast Yarrowia lipolytica.Applied microbiology and biotechnology · 2025Article
- Decompartmentalization of the yeast mitochondrial metabolism to improve chemical production in Issatchenkia orientalis.Nature communications · 2025Article
- Fully exposed PtNature communications · 2025Article
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Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
Abstract
Succinic acid (SA) is an important C4-dicarboxylic acid. Microbial production of SA at low pH results in low purification costs and hence good overall process economics. However, redox imbalances limited SA biosynthesis from glucose via the reductive tricarboxylic acid (TCA) cycle in yeast. Here, we engineer the strictly aerobic yeast Yarrowia lipolytica for efficient SA production without pH control. Introduction of the reductive TCA cycle into the cytosol of a succinate dehydrogenase-disrupted yeast strain causes arrested cell growth. Although adaptive laboratory evolution restores cell growth, limited NADH supply restricts SA production. Reconfiguration of the reductive SA biosynthesis pathway in the mitochondria through coupling the oxidative and reductive TCA cycle for NADH regeneration results in improved SA production. In pilot-scale fermentation, the engineered strain produces 111.9 g/L SA with a yield of 0.79 g/g glucose within 62 h. This study paves the way for industrial production of biobased SA.
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