Evidence map›Paper›PMID 38112461›Full record

ArticleJournal of applied biomedicine2023

Homospisulosine induced apoptosis in cervical carcinoma cells is associated with phosphorylation of Bcl-2 and up-regulation of p27/Kip1.

Martina Bago Pilatova, Natalia Nosalova, Gabriela Ockajakova, Martin Kello, Klaudia Kotorova, Peter Takac, Peter Petik, Peter Bohus, Kvetoslava Stankova, Miroslava Martinkova and 1 more

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In one paragraph

Article in Journal of applied biomedicine, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 2 countries.

Martina Bago PilatovaPavol Jozef Safarik University, Faculty of Medicine, Department of Pharmacology, Kosice, Slovak Republic.
Natalia NosalovaPavol Jozef Safarik University, Faculty of Medicine, Department of Pharmacology, Kosice, Slovak Republic.
Gabriela OckajakovaPavol Jozef Safarik University, Faculty of Medicine, Department of Pharmacology, Kosice, Slovak Republic.
Martin KelloPavol Jozef Safarik University, Faculty of Medicine, Department of Pharmacology, Kosice, Slovak Republic.
Klaudia KotorovaPavol Jozef Safarik University, Faculty of Medicine, Department of Pharmacology, Kosice, Slovak Republic.
Peter TakacUniversity of Veterinary Medicine and Pharmacy, Department of Pharmacology and Toxicology, Kosice, Slovak Republic.
Peter PetikPavol Jozef Safarik University, Faculty of Medicine, Department of Pathology, Kosice, Slovak Republic.
Peter BohusPavol Jozef Safarik University, Faculty of Medicine, Department of Pathology, Kosice, Slovak Republic.
Kvetoslava StankovaPavol Jozef Safarik University, Faculty of Science, Department of Organic Chemistry, Institute of Chemical Sciences, Kosice, Slovak Republic.
Miroslava MartinkovaPavol Jozef Safarik University, Faculty of Science, Department of Organic Chemistry, Institute of Chemical Sciences, Kosice, Slovak Republic.
Roman MezencevGeorgia Institute of Technology, School of Biological Sciences, Atlanta, GA, USA.
University of Pavol Jozef Šafárik · SKGeorgia Institute of Technology · USUniversity of Veterinary Medicine in Košice · SK

Funding

Operational Programme Integrated Infrastructure 313011V455Operational Programme Integrated Infrastructure ITMS2014Slovak Grant Agency for Science VEGA No 1/0278/23Slovak Grant Agency for Science VEGA No 1/0539/21Slovak Grant Agency for Science VEGA No 1/0653/19
6 · The paper itself

Abstract

Spisulosine (1-deoxysphinganine) is a sphingoid amino alcohol isolated from the sea clams that showed potent antiproliferative activity against a broad spectrum of solid tumors but failed in clinical trials due to neurotoxicity. However, its structural similarity to other bioactive sphingoids, interesting mode of action, and appreciable potency against cancer cells make it a suitable lead for future anticancer drug development. The present study was conducted to elucidate mechanisms of the antiproliferative/cytotoxic effects of newly synthesized spisulosine analog homospisulosine (KP7). The evaluation was performed on cervical carcinoma cells, representing an in vitro model of one of the most common cancer types and a significant worldwide cause of women's cancer mortality. Treatment with homospisulosine (2.0 μM) for 24, 48, and 72 h significantly inhibited the growth of HeLa cells in vitro and induced apoptosis detectable by DNA fragmentation, externalization of phosphatidylserine, dissipation of mitochondrial membrane potential, activation of caspase-3 and cleavage of PARP. In addition, treating HeLa cells with spisulosine increased p27 and Bcl-2 on protein levels and phosphorylation of Bcl-2 on Ser70 residue. These results support the potential for spisulosine analogs represented here by homospisulosine for future therapeutic development.

Indexed as

Antineoplastic AgentsCarcinomaApoptosisFemaleHeLa CellsHumansPhosphorylationUp-RegulationAntineoplastic AgentsApoptosisCancerCeramideHomospisulosineMarineSphingoid basesSphingosine-1-phosphateSpisulosine

Identifiers

PMID38112461
OpenAlexW4389087415

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.