ArticlePNAS nexus2023
Rapid assessment of changes in phage bioactivity using dynamic light scattering.
Article in PNAS nexus, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 20 citations in OpenAlex.
- Single-step monolithic chromatography efficiently purifies diverse Pseudomonas aeruginosa phages with therapeutic-grade endotoxin reduction.Npj viruses · 2026Article
- Whole-body distribution of threeAntimicrobial agents and chemotherapy · 2026Article
- Automated Covalent Microcontact Bioprinting of Lubricant-Infused Microarrays for Biological Assays.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Unraveling the Inactivation Mechanisms of Human Adenovirus 2 in Sunlight Disinfection: Synergism between Direct and Indirect Pathways.Environmental science & technology · 2025Article
- Preparation and pharmacokinetic evaluation of Staphylococcus phage COP-80B for treatment of periprosthetic joint infections in a mouse model.Virus research · 2025Article
- Dosing and Delivery of Bacteriophage Therapy In a Murine Wound Infection Model.bioRxiv : the preprint server for biology · 2025Article
- Extreme diversity of phage amplification rates and phage-antibiotic interactions revealed by PHORCE.PLoS biology · 2025Article
- Design, optimization, and inference of biphasic decay of infectious virus particles.Journal of theoretical biology · 2025Article
- Bacteriophage therapy for multidrug-resistant infections: current technologies and therapeutic approaches.The Journal of clinical investigation · 2025Review
- Degradation of Preformed Gram-Positive and Gram-Negative Bacterial Biofilms Using Disintegrated and Intact Phages.PHAGE (New Rochelle, N.Y.) · 2025Article
- Real-time monitoring by interferometric light microscopy of phage suspensions for personalised phage therapy.Scientific reports · 2024Article
- The contribution of neutrophils to bacteriophage clearance and pharmacokinetics in vivo.JCI insight · 2024Article
- Engineering ofCells · 2023Article
Corrections and comments
- Erratum issued
- Update of
Authors and funding
20 authors at 5 institutions in 3 countries.
Funding
Abstract
Extensive efforts are underway to develop bacteriophages as therapies against antibiotic-resistant bacteria. However, these efforts are confounded by the instability of phage preparations and a lack of suitable tools to assess active phage concentrations over time. In this study, we use dynamic light scattering (DLS) to measure changes in phage physical state in response to environmental factors and time, finding that phages tend to decay and form aggregates and that the degree of aggregation can be used to predict phage bioactivity. We then use DLS to optimize phage storage conditions for phages from human clinical trials, predict bioactivity in 50-y-old archival stocks, and evaluate phage samples for use in a phage therapy/wound infection model. We also provide a web application (Phage-Estimator of Lytic Function) to facilitate DLS studies of phages. We conclude that DLS provides a rapid, convenient, and nondestructive tool for quality control of phage preparations in academic and commercial settings.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.