Evidence map›Paper›PMID 38111309›Full record

ArticleJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc2024

Optimization of a foot-and-mouth disease virus Southern African Territories-specific solid-phase competitive ELISA for small ruminant serum samples.

LaToya Seoke, Geoffrey T Fosgate, Pamela A Opperman, Refiloe P Malesa, David D Lazarus, Mohamed M Sirdar, Livio Heath

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.7field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 2 countries.

LaToya SeokeDepartment of Production Animal Studies, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa.ORCID 0000-0001-9854-6535
Geoffrey T FosgateDepartment of Production Animal Studies, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa.ORCID 0000-0002-9432-0042
Pamela A OppermanDepartment of Production Animal Studies, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa.
Refiloe P MalesaTransboundary Animal Diseases Program, Onderstepoort Veterinary Research, Agricultural Research Council, Onderstepoort, South Africa.
David D LazarusEpidemiology and Training Program of Onderstepoort Veterinary Research, Agricultural Research Council, Onderstepoort, South Africa.
Mohamed M SirdarWorld Organisation for Animal Health Sub-Regional Representative for Southern Africa, Gaborone, Botswana.
Livio HeathTransboundary Animal Diseases Program, Onderstepoort Veterinary Research, Agricultural Research Council, Onderstepoort, South Africa.
Southern African Development Community · BW

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We optimized and verified a single-spot solid-phase competitive ELISA (ss-SPCE) to detect antibodies against structural proteins of Southern African Territories (SAT) serotypes of foot-and-mouth disease virus (FMDV) in small ruminants. Sera from goats vaccinated and experimentally challenged with a SAT1 FMDV pool were tested in duplicate at 4 dilutions (1:10, 1:15, 1:22.5, 1:33.8) to optimize the assay. To assess the performance of the assay in naturally infected animals, we evaluated 316 goat and sheep field sera collected during active SAT2 outbreaks. Relative to results of the virus neutralization test, the optimal serum dilution and cutoff percentage inhibition (PI) were 1:15 and 50%, respectively. At these values, the Spearman rank correlation coefficient was 0.85 (

Indexed as

Foot-and-Mouth DiseaseFoot-and-Mouth Disease VirusGoat DiseasesSheep DiseasesAnimalsAntibodies, ViralEnzyme-Linked Immunosorbent AssayGoatsSerogroupSheepAntibodies, Viralfoot-and-mouth diseaseserologysmall ruminantsSouthern African Territoriestest optimization

Identifiers

PMID38111309
PMCPMC10929636
OpenAlexW4389953544

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.