Evidence map›Paper›PMID 38110595›Full record

ArticleScientific reports2023

Discovery of novel neutral glycosphingolipids in cereal crops: rapid profiling using reversed-phased HPLC-ESI-QqTOF with parallel reaction monitoring.

Dingyi Yu, Berin A Boughton, Thusitha W T Rupasinghe, Camilla B Hill, Cornelia Herrfurth, Patricia Scholz, Ivo Feussner, Ute Roessner

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 6 institutions in 3 countries.

Dingyi YuSchool of BioSciences, University of Melbourne, Parkville, VIC, 3010, Australia.
Berin A BoughtonSchool of BioSciences, University of Melbourne, Parkville, VIC, 3010, Australia. b.boughton@latrobe.edu.au.
Thusitha W T RupasingheSchool of BioSciences, University of Melbourne, Parkville, VIC, 3010, Australia.
Camilla B HillSchool of BioSciences, University of Melbourne, Parkville, VIC, 3010, Australia.
Cornelia HerrfurthDepartment of Plant Biochemistry, Albrecht-Von-Haller-Institute for Plant Sciences, University of Goettingen, Justus-Von-Liebig Weg 11, 37077, Goettingen, Germany.
Patricia ScholzDepartment of Plant Biochemistry, Albrecht-Von-Haller-Institute for Plant Sciences, University of Goettingen, Justus-Von-Liebig Weg 11, 37077, Goettingen, Germany.
Ivo FeussnerDepartment of Plant Biochemistry, Albrecht-Von-Haller-Institute for Plant Sciences, University of Goettingen, Justus-Von-Liebig Weg 11, 37077, Goettingen, Germany.
Ute RoessnerSchool of BioSciences, University of Melbourne, Parkville, VIC, 3010, Australia.
The University of Melbourne · AUUniversity of Göttingen · DEAgriculture and Food · AUAustralian National University · AUÉcole Normale Supérieure de Lyon · FRSt Vincents Institute of Medical Research · AU

Funding

Australian Research Council FT130100326University of Melbourne Dyson Fellowship
6 · The paper itself

Abstract

This study explores the sphingolipid class of oligohexosylceramides (OHCs), a rarely studied group, in barley (Hordeum vulgare L.) through a new lipidomics approach. Profiling identified 45 OHCs in barley (Hordeum vulgare L.), elucidating their fatty acid (FA), long-chain base (LCB) and sugar residue compositions; and was accomplished by monophasic extraction followed by reverse-phased high performance liquid chromatography electrospray ionisation quadrupole-time-of-flight tandem mass spectrometry (HPLC-ESI-QqTOF-MS/MS) employing parallel reaction monitoring (PRM). Results revealed unknown ceramide species and highlighted distinctive FA and LCB compositions when compared to other sphingolipid classes. Structurally, the OHCs featured predominantly trihydroxy LCBs associated with hydroxylated FAs and oligohexosyl residues consisting of two-five glucose units in a linear 1 → 4 linkage. A survey found OHCs in tissues of major cereal crops while noting their absence in conventional dicot model plants. This study found salinity stress had only minor effects on the OHC profile in barley roots, leaving questions about their precise functions in plant biology unanswered.

Indexed as

Neutral GlycosphingolipidsTandem Mass SpectrometryChromatography, High Pressure LiquidEdible GrainFatty AcidsSpectrometry, Mass, Electrospray IonizationSphingolipidsFatty AcidsNeutral GlycosphingolipidsSphingolipids

Identifiers

PMID38110595
PMCPMC10728066
OpenAlexW4389903123

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.