Evidence map›Paper›PMID 38110570›Full record

ArticleParasitology research2023

Immunisation with Neospora caninum subunits rsNcSAG4 and rsNcGRA1 (NcSAG4 and NcGRA1 epitopes construct) in BALB/c mice: the profile of the immune response and controlling the vertical transmission.

Cintia Fernandes Fidelis, Leandro Silva de Araújo, Pablo A Prates-Patarroyo, Karlos H Martins-Kalks, Leandro Licursi de Oliveira, Marlene Isabel Vargas Viloria, Gabriel A Tafur-Gómez, Joaquín Hernán Patarroyo Salcedo

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Parasitology research, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 84% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Cintia Fernandes FidelisLaboratório de Biologia e Controle de Hematozoários e Vetores, Departamento de Veterinária, Instituto de Biotecnologia Aplicada à Agropecuária - BIOAGRO, Universidade Federal de Viçosa - UFV, CEP, Viçosa, MG, 36570-900, Brazil.ORCID https://orcid.org/0000-0002-7014-4136
Leandro Silva de AraújoLaboratório de Biologia e Controle de Hematozoários e Vetores, Departamento de Veterinária, Instituto de Biotecnologia Aplicada à Agropecuária - BIOAGRO, Universidade Federal de Viçosa - UFV, CEP, Viçosa, MG, 36570-900, Brazil.
Pablo A Prates-PatarroyoLaboratório de Biologia e Controle de Hematozoários e Vetores, Departamento de Veterinária, Instituto de Biotecnologia Aplicada à Agropecuária - BIOAGRO, Universidade Federal de Viçosa - UFV, CEP, Viçosa, MG, 36570-900, Brazil.
Karlos H Martins-KalksLaboratório de Biologia e Controle de Hematozoários e Vetores, Departamento de Veterinária, Instituto de Biotecnologia Aplicada à Agropecuária - BIOAGRO, Universidade Federal de Viçosa - UFV, CEP, Viçosa, MG, 36570-900, Brazil.
Leandro Licursi de OliveiraLaboratório de Imunoquímica e Glicobiologia, Departamento de Biologia Geral, Universidade Federal de Viçosa - UFV, CEP, Viçosa, MG, 36570-900, Brazil.ORCID https://orcid.org/0000-0003-4353-7011
Marlene Isabel Vargas ViloriaLaboratório de Biologia e Controle de Hematozoários e Vetores, Departamento de Veterinária, Instituto de Biotecnologia Aplicada à Agropecuária - BIOAGRO, Universidade Federal de Viçosa - UFV, CEP, Viçosa, MG, 36570-900, Brazil.ORCID https://orcid.org/0000-0002-3045-3241
Gabriel A Tafur-GómezUniversidad de Ciencias Aplicadas y Ambientales - U.D.C.A, Bogotá, 111166, Colombia. gatafur@udca.edu.co.ORCID https://orcid.org/0000-0003-2829-6256
Joaquín Hernán Patarroyo SalcedoLaboratório de Biologia e Controle de Hematozoários e Vetores, Departamento de Veterinária, Instituto de Biotecnologia Aplicada à Agropecuária - BIOAGRO, Universidade Federal de Viçosa - UFV, CEP, Viçosa, MG, 36570-900, Brazil. jpatarro@ufv.br.ORCID https://orcid.org/0000-0001-9236-7034
Universidade Federal de Viçosa · BRUniversity of Applied and Environmental Sciences · CO

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Neospora caninum is an apicomplexan protozoan that causes neosporosis, which has a high economic impact on cattle herds with no available vaccine. During infection, the secretion of dense granules and the expression of surface antigens play an important role in hosting immunomodulation. However, some epitopes of those antigens are immunogenic, and using these fractions could improve the subunit antigens in vaccine design. This study evaluates the recombinant peptides rsNcGRA1 and rsNcSAG4 derived from NcGRA1 and NcSAG4 native antigens as vaccine candidates produced by a fermentative process in the yeast culture system of Komagataella phaffii strain Km71, confirmed by colony PCR, SDS-PAGE, and western blotting. The assay was conducted in BALB/c mice using the peptides at low (25 μg) and standard (50 μg) dosages in monovalent and combined administrations at three time points with saponin as an adjuvant assessing the immunogenicity by antibodies response and cytokine production. We challenge the females after pregnancy confirmation using 2 × 10

Indexed as

CoccidiosisNeosporaProtozoan VaccinesAnimalsAntibodies, ProtozoanAntigens, ProtozoanChlorocebus aethiopsCytokinesEpitopesFemaleImmunityInfectious Disease Transmission, VerticalMiceMice, Inbred BALB CPersistent InfectionPregnancyAntibodies, ProtozoanAntigens, ProtozoanCytokinesEpitopesProtozoan VaccinesImmunogenNcGRA1NcSAG4Neospora caninumRecombinant peptidesSubunit vaccines

Identifiers

PMID38110570
PMCPMC10728228
OpenAlexW4389914377

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.