Evidence map›Paper›PMID 38110036›Full record

ArticleThe Journal of biological chemistry2024

Molecular mechanisms underlying the structural diversity of rhamnose-rich cell wall polysaccharides in lactococci.

Hugo Guérin, Pascal Courtin, Alain Guillot, Christine Péchoux, Jennifer Mahony, Douwe van Sinderen, Saulius Kulakauskas, Christian Cambillau, Thierry Touzé, Marie-Pierre Chapot-Chartier

Open access · goldAbstract read
In one paragraph

Article in The Journal of biological chemistry, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.8field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 4 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 2 countries.

Hugo GuérinUniversité Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Jouy-en-Josas, France.
Pascal CourtinUniversité Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Jouy-en-Josas, France.
Alain GuillotUniversité Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Jouy-en-Josas, France.
Christine PéchouxUniversité Paris-Saclay INRAE, AgroParisTech, GABI, Jouy-en-Josas, France.
Jennifer MahonySchool of Microbiology and APC Microbiome Ireland, University College Cork, Cork, Ireland.
Douwe van SinderenSchool of Microbiology and APC Microbiome Ireland, University College Cork, Cork, Ireland.
Saulius KulakauskasUniversité Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Jouy-en-Josas, France.
Christian CambillauSchool of Microbiology and APC Microbiome Ireland, University College Cork, Cork, Ireland; Laboratoire d'Ingénierie des Systèmes Macromoléculaires (LISM), Institut de Microbiologie, Bioénergies et Biotechnologie (IMM), Aix-Marseille Université - CNRS, UMR 7255, Marseille, France.
Thierry TouzéUniversité Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Gif-sur-Yvette, France.
Marie-Pierre Chapot-ChartierUniversité Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Jouy-en-Josas, France. Electronic address: marie-pierre.chapot-chartier@inrae.fr.
AgroParisTech · FRCentre National de la Recherche Scientifique · FRUniversity College Cork · IE

Funding

ChimeraX -- Next Generation Visualization and Analysis Software for Multiscale ModelingR01GM129325 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI FERRIN, THOMAS E · 2018 to 2025
$5.2M
NIGMS NIH HHS R01 GM129325
6 · The paper itself

Abstract

In Gram-positive bacteria, cell wall polysaccharides (CWPS) play critical roles in bacterial cell wall homeostasis and bacterial interactions with their immediate surroundings. In lactococci, CWPS consist of two components: a conserved rhamnan embedded in the peptidoglycan layer and a surface-exposed polysaccharide pellicle (PSP), which are linked together to form a large rhamnose-rich CWPS (Rha-CWPS). PSP, whose structure varies from strain to strain, is a receptor for many bacteriophages infecting lactococci. Here, we examined the first two steps of PSP biosynthesis, using in vitro enzymatic tests with lipid acceptor substrates combined with LC-MS analysis, AlfaFold2 modeling of protein 3D-structure, complementation experiments, and phage assays. We show that the PSP repeat unit is assembled on an undecaprenyl-monophosphate (C

Indexed as

Cell WallLactococcusPolysaccharides, BacterialRhamnoseBacterial ProteinsBacteriophagesDeoxy SugarsGlycosyltransferasesLipidsMannansPeptidoglycanProtein ConformationSubstrate SpecificityBacterial ProteinsDeoxy SugarsGlycosyltransferasesLipidsMannansPeptidoglycanPolysaccharides, BacterialrhamnanRhamnosebacteriophagecell wallglycosyltransferasegram-positive bacteriaLactococcuspolysaccharide

Identifiers

PMID38110036
PMCPMC10821137
OpenAlexW4389822925

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.