Evidence map›Paper›PMID 38105945›Full record

ArticlebioRxiv : the preprint server for biology2023

Nanoscale photobiotinylation, pulldown and sequencing of region-specific DNA from intact cells.

Thomas C Roberts, Max Kushner, Jack C Crowley, Abdullah Ozer, Juan Wang, Judhajeet Ray, John T Lis, Warren R Zipfel

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Thomas C RobertsSmith School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY USA 14853.
Max KushnerField of Biophysics, Cornell University, Ithaca NY USA 14853.
Jack C CrowleyApplied and Engineering Physics, Cornell University, Ithaca, NY USA 14853.
Abdullah OzerDepartment of Molecular Biology and Genetics, Cornell University, Ithaca NY USA 14853.ORCID 0000-0001-6861-2853
Juan WangField of Biophysics, Cornell University, Ithaca NY USA 14853.
Judhajeet RayDepartment of Molecular Biology and Genetics, Cornell University, Ithaca NY USA 14853.ORCID 0000-0003-1524-2603
John T LisDepartment of Molecular Biology and Genetics, Cornell University, Ithaca NY USA 14853.ORCID 0000-0003-1201-9406
Warren R ZipfelSmith School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY USA 14853.ORCID 0000-0003-2640-329X

Funding

Distance-Hi-C: Creating Photo Activated X-linkers To Define Nuclear ArchitectureU01HL129958 · NHLBI · CORNELL UNIVERSITY · PI LIN, HENING, LIS, JOHN T · 2015 to 2019
$3.6M
Femto-seq: Targeted photo-biotinylation, pulldown and sequencing of locus and region-specific DNA from femtoliter volumes within individual cellsR01GM145934 · NIGMS · CORNELL UNIVERSITY · PI WARREN R ZIPFEL · 2023 to 2026
$1.4M
NHLBI NIH HHS U01 HL129958NIGMS NIH HHS R01 GM145934
6 · The paper itself

Abstract

Femto-seq is a novel nanoscale optical method that can be used to obtain DNA sequence information from targeted regions around a specific locus or other nuclear regions of interest. Two-photon excitation is used to photobiotinylate femtoliter volumes of chromatin within the nucleus, allowing for subsequent isolation and sequencing of DNA, and bioinformatic mapping of any nuclear region of interest in a select set of cells from a heterogenous population.

Identifiers

PMID38105945
PMCPMC10723339

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.