Evidence map›Paper›PMID 38105942›Full record

ArticlebioRxiv : the preprint server for biology2023

AP-2α/AP-2β transcription factors are key regulators of epidermal homeostasis.

Hui Zhang, Jackelyn Raymundo, Kathleen E Daly, Wenjuan Zhu, Bill Senapati, Alexander G Marneros

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Hui ZhangCutaneous Biology Research Center, Department of Dermatology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, 02129, USA.ORCID 0000-0002-4936-4791
Jackelyn RaymundoCutaneous Biology Research Center, Department of Dermatology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, 02129, USA.ORCID 0009-0002-2725-0715
Kathleen E DalyCutaneous Biology Research Center, Department of Dermatology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, 02129, USA.ORCID 0009-0006-1346-8747
Wenjuan ZhuCardiovascular Institute, Stanford University, Stanford, CA, 94305, USA.ORCID 0000-0003-2724-4825
Bill SenapatiCutaneous Biology Research Center, Department of Dermatology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, 02129, USA.ORCID 0009-0007-5199-2299
Alexander G MarnerosCutaneous Biology Research Center, Department of Dermatology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, 02129, USA.ORCID 0000-0003-3866-020X
Harvard University · USCardiovascular Institute of the South · US

Funding

Cellular and molecular mechanisms of AIM2 and NLRP3 inflammasome activation in age-related macular degenerationR01EY033709 · NEI · MASSACHUSETTS GENERAL HOSPITAL · PI Alexander Georg Marneros · 2023 to 2026
$2.0M
Functional characterization of a novel key regulator of the distal nephron whose deficiency leads to renal fibrosis and cyst formationR01DK118134 · NIDDK · MASSACHUSETTS GENERAL HOSPITAL · PI MARNEROS, ALEXANDER GEORG · 2019 to 2023
$1.7M
Proangiogenic M2-type macrophages and choroidal neovascularizationR01EY033360 · NEI · MASSACHUSETTS GENERAL HOSPITAL · PI Alexander Georg Marneros · 2022 to 2026
$1.7M
Mechanisms controlling distal nephron maturationR01DK121178 · NIDDK · MASSACHUSETTS GENERAL HOSPITAL · PI MARNEROS, ALEXANDER GEORG · 2019 to 2022
$1.4M
NEI NIH HHS R01 EY033360NEI NIH HHS R01 EY033709NIDDK NIH HHS R01 DK118134NIDDK NIH HHS R01 DK121178
6 · The paper itself

Abstract

AP-2 transcription factors regulate ectodermal development but their roles for epidermal homeostasis in the adult skin are unknown. We find that AP-2α is the predominant AP-2 family member in adult epidermis, followed by AP-2β. Through inactivation of AP-2α, AP-2β, or both in keratinocytes we assessed the effects of a gradient of epidermal AP-2 activity on skin function. We find that (1) loss of AP-2β in keratinocytes is compensated for by AP-2α, (2) loss of AP-2α impairs terminal keratinocyte differentiation and hair morphogenesis, and (3) the combined loss of AP-2α/AP-2β results in more severe skin and hair abnormalities. Keratinocyte differentiation defects precede a progressive neutrophilic skin inflammation. Inducible inactivation of AP-2α/AP-2β in the adult phenocopies these manifestations. Transcriptomic analyses of epidermis lacking AP-2α or AP-2α/AP-2β in keratinocytes demonstrate a terminal keratinocyte differentiation defect with upregulation of alarmin keratins and of several immune pathway regulators. Moreover, our analyses suggest a key role of loss of AP-2α-dependent gene expression of CXCL14 and KRT15 as an early pathogenic event towards the manifestation of skin inflammation. Thus, AP-2α/AP-2β are critical regulators of epidermal homeostasis in the adult skin.

Identifiers

PMID38105942
PMCPMC10723278
OpenAlexW4389297076

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.