ArticlePlant physiology2024
OXIDOSQUALENE CYCLASE 1 and 2 influence triterpene biosynthesis and defense in Nicotiana attenuata.
Article in Plant physiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 20 citations in OpenAlex.
- Genome-Wide Identification and Expression Profiling of the Oxidosqualene Cyclase Gene Family inCurrent issues in molecular biology · 2026Article
- Chromosome-level genome assembly of Tamarindus indica provides new insights into the evolution of triterpenes and tartaric acid biosynthetic pathway.Molecular horticulture · 2026Article
- Structural classification, biosynthesis, metabolic engineering and ecological functions for the terpenes produced in tobacco.Frontiers in plant science · 2026Review
- Article
- Identification of 2,3-oxidosqualene cyclase gene inHorticulture research · 2025Article
- Integrated multi-omics reveals flavonoid-dominated defense strategies in Coptis chinensis under Fusarium root rot infection.BMC plant biology · 2025Article
- The emergence and loss of cyclic peptides inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Discovery and characterisation of terpenoid biosynthesis enzymes from Daphniphyllum macropodum.BMC plant biology · 2025Article
- Expansion of the Stereochemical Space of Triterpenes by Mining Noncanonical Oxidosqualene Cyclases Across the Diversity of Green Plants.Journal of the American Chemical Society · 2025Article
- Roles of three cytochrome P450 monooxygenases in triterpene biosynthesis and their potential impact on growth and development.Plant physiology · 2024Article
- Triterpene tales: Two genes involved in Nicotiana attenuata herbivore defense.Plant physiology · 2024Article
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3 authors at 1 institution in 1 country.
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Abstract
Triterpenes are a class of bioactive compounds with diverse biological functions, playing pivotal roles in plant defense against biotic stressors. Oxidosqualene cyclases (OSCs) serve as gatekeepers in the biosynthesis of triterpenes. In this study, we utilized a Nicotiana benthamiana heterologous expression system to characterize NaOSC1 from Nicotiana attenuata as a multifunctional enzyme capable of synthesizing lupeol, dammarenediol II, 3-alpha,20-lupanediol, and 7 other triterpene scaffolds. We also demonstrated that NaOSC2 is, in contrast, a selective enzyme, producing only the β-amyrin scaffold. Through virus-induced gene silencing and in vitro toxicity assays, we elucidated the roles of NaOSC1 and NaOSC2 in the defense of N. attenuata against Manduca sexta larvae. Metabolomic and feature-based molecular network analyses of leaves with silenced NaOSC1 and NaOSC2 unveiled 3 potential triterpene glycoside metabolite clusters. Interestingly, features identified as triterpenes within these clusters displayed a significant negative correlation with larval mass. Our study highlights the pivotal roles of NaOSC1 and NaOSC2 from N. attenuata in the initial steps of triterpene biosynthesis, subsequently influencing defense against M. sexta through the modulation of downstream triterpene glycoside compounds.
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