ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2024
Genotyping of European Toxoplasma gondii strains by a new high-resolution next-generation sequencing-based method.
Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed, 17 citations in OpenAlex.
- Toxoplasmosis Acquired in French Guiana in Immunocompetent Adults: Case Series From 2002 to 2019, With a Focus on Outpatients.Open forum infectious diseases · 2026Article
- Evolution ofJournal of parasitology research · 2026Review
- Seroprevalence and genetic characterization of Toxoplasma gondii in hunted wild boars (Sus scrofa) from Croatia.Parasitology research · 2025Article
- Identification of a mouse-virulent recombinant type I/III Toxoplasma gondii strain in liver cytology of an immunosuppressed cat infected with FeLV-C subgroup.Veterinary research communications · 2025Article
- Isolation, genotyping and phenotypingFood and waterborne parasitology · 2025Review
- Design, development, and testing of a new multi-locus sequence typing scheme for the zoonotic pathogenCurrent research in parasitology & vector-borne diseases · 2025Article
- The importance of translational research in the study of ocular toxoplasmosis: insights from the 17th International Congress on Toxoplasmosis 2024.FEMS microbes · 2025Article
- Role of wild birds in the circulation ofFrontiers in veterinary science · 2025Article
- Multiple Typing Approach to CharacterizeAnimals : an open access journal from MDPI · 2024Article
- Rapid genotyping of Toxoplasma gondii isolates via Nanopore-based multi-locus sequencing.AMB Express · 2024Article
- A combination of GRA3, GRA6 and GRA7 peptides offer a useful tool for serotyping type II and IIIFrontiers in cellular and infection microbiology · 2024Article
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Authors and funding
35 authors at 17 institutions in 14 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeA new high-resolution next-generation sequencing (NGS)-based method was established to type closely related European type II Toxoplasma gondii strains.
methodsT. gondii field isolates were collected from different parts of Europe and assessed by whole genome sequencing (WGS). In comparison to ME49 (a type II reference strain), highly polymorphic regions (HPRs) were identified, showing a considerable number of single nucleotide polymorphisms (SNPs). After confirmation by Sanger sequencing, 18 HPRs were used to design a primer panel for multiplex PCR to establish a multilocus Ion AmpliSeq typing method. Toxoplasma gondii isolates and T. gondii present in clinical samples were typed with the new method. The sensitivity of the method was tested with serially diluted reference DNA samples.
resultsAmong type II specimens, the method could differentiate the same number of haplotypes as the reference standard, microsatellite (MS) typing. Passages of the same isolates and specimens originating from abortion outbreaks were identified as identical. In addition, seven different genotypes, two atypical and two recombinant specimens were clearly distinguished from each other by the method. Furthermore, almost all SNPs detected by the Ion AmpliSeq method corresponded to those expected based on WGS. By testing serially diluted DNA samples, the method exhibited a similar analytical sensitivity as MS typing.
conclusionThe new method can distinguish different T. gondii genotypes and detect intra-genotype variability among European type II T. gondii strains. Furthermore, with WGS data additional target regions can be added to the method to potentially increase typing resolution.
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