Evidence map›Paper›PMID 38099960›Full record

ArticleThe European physical journal. E, Soft matter2023

Lipids with negative spontaneous curvature decrease the solubility of the cancer drug paclitaxel in liposomes.

Victoria Steffes, Scott MacDonald, John Crowe, Meena Murali, Kai K Ewert, Youli Li, Cyrus R Safinya

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The European physical journal. E, Soft matter, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.5field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. An overview of pyridazin-3(2Future medicinal chemistry · 2024
    Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Victoria SteffesMaterials Department, University of California, Santa Barbara, CA, 93106, USA.
Scott MacDonaldPhysics Department, University of California, Santa Barbara, CA, 93106, USA.
John CrowePhysics Department, University of California, Santa Barbara, CA, 93106, USA.
Meena MuraliMolecular, Cellular, and Developmental Biology Department, University of California, Santa Barbara, CA, 93106, USA.
Kai K EwertMaterials Department, University of California, Santa Barbara, CA, 93106, USA.
Youli LiMaterials Research Laboratory, University of California, Santa Barbara, CA, 93106, USA.
Cyrus R SafinyaMaterials Department, University of California, Santa Barbara, CA, 93106, USA. cyrussafinya@ucsb.edu.
University of California, Santa Barbara · US

Funding

Functionalized Lipid Carriers for Nucleic-Acid and Drug TherapeuticsR01GM130769 · NIGMS · UNIVERSITY OF CALIFORNIA SANTA BARBARA · PI SAFINYA, CYRUS R · 2018 to 2021
$1.4M
NIGMS NIH HHS R01 GM130769NIGMS NIH HHS R01GM130769
6 · The paper itself

Abstract

Paclitaxel (PTX) is a hydrophobic small-molecule cancer drug that loads into the membrane (tail) region of lipid carriers such as liposomes and micelles. The development of improved lipid-based carriers of PTX is an important objective to generate chemotherapeutics with fewer side effects. The lipids 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) and glyceryl monooleate (GMO) show propensity for fusion with other lipid membranes, which has led to their use in lipid vectors of nucleic acids. We hypothesized that DOPE and GMO could enhance PTX delivery to cells through a similar membrane fusion mechanism. As an important measure of drug carrier performance, we evaluated PTX solubility in cationic liposomes containing GMO or DOPE. Solubility was determined by time-dependent kinetic phase diagrams generated from direct observations of PTX crystal formation using differential-interference-contrast optical microscopy. Remarkably, PTX was much less soluble in these liposomes than in control cationic liposomes containing univalent cationic lipid 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) and 1,2-dioleoyl-sn-glycero-3-phosphatidylcholine (DOPC), which are not fusogenic. In particular, PTX was not substantially soluble in GMO-based cationic liposomes. The fusogenicity of DOPE and GMO is related to the negative spontaneous curvature of membranes containing these lipids, which drives formation of nonlamellar self-assembled phases (inverted hexagonal or gyroid cubic). To determine whether PTX solubility is governed by lipid membrane structure or by local intermolecular interactions, we used synchrotron small-angle X-ray scattering. To increase the signal/noise ratio, we used DNA to condense the lipid formulations into lipoplex pellets. The results suggest that local intermolecular interactions are of greater importance and that the negative spontaneous curvature-inducing lipids DOPE and GMO are not suitable components of liposomal carriers for PTX delivery.

Indexed as

Antineoplastic AgentsNeoplasmsLiposomesMicellesPaclitaxelSolubilityAntineoplastic AgentsLiposomesMicellesPaclitaxel

Identifiers

PMID38099960
PMCPMC10802834
OpenAlexW4389782885

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.