ArticleFrontiers in veterinary science2023
Reviving vacuum-dried encapsulated ram spermatozoa via ICSI after 2 years of storage.
Article in Frontiers in veterinary science, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed, 10 citations in OpenAlex.
- Freeze-Drying and Room Temperature Preservation of Mammalian Sperm.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Reproductive Sciences to the Rescue of Wild Animal Species.Advances in experimental medicine and biology · 2026Review
- Improved birth rates via rehydration of mouse freeze-dried spermatozoa using high-temperature ultrapure water.PloS one · 2026Article
- The challenges and prospects of mammalian reproduction in extraterrestrial environments.The Journal of reproduction and development · 2026Review
- Monosodium glutamate enhances freeze-dried mouse sperm quality during room-temperature storage.Scientific reports · 2025Article
- Can Humanity Thrive Beyond the Galaxy?The Journal of reproduction and development · 2025Review
- Method for long-term room temperature storage of mouse freeze-dried sperm.Scientific reports · 2025Article
Corrections and comments
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Authors and funding
12 authors at 7 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Introduction: Freeze-drying techniques give alternative preservation mammalian spermatozoa without liquid nitrogen. However, most of the work has been conducted in the laboratory mouse, while little information has been gathered on large animals that could also benefit from this kind of storage. Methods: This work adapted a technique known as vacuum-drying encapsulation (VDE), originally developed for nucleic acid conservation in anhydrous state, to ram spermatozoa, and compared it to canonical lyophilization (FD), testing long-term storage at room temperature (RT) and 4°C. Results and discussion: The results demonstrated better structural stability, namely lipid composition and DNA integrity, in VDE spermatozoa than FD ones, with outcomes at RT storage comparable to 4°C. Likewise, in VDE the embryonic development was higher than in FD samples (12.8% vs. 8.7%,
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Registered trials
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