Evidence map›Paper›PMID 38097537›Full record

ArticleLight, science & applications2023

Quantitatively mapping local quality of super-resolution microscopy by rolling Fourier ring correlation.

Weisong Zhao, Xiaoshuai Huang, Jianyu Yang, Liying Qu, Guohua Qiu, Yue Zhao, Xinwei Wang, Deer Su, Xumin Ding, Heng Mao and 7 more

Abstract read
In one paragraph

Article in Light, science & applications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Weisong Zhao *Innovation Photonics and Imaging Center, School of Instrumentation Science and Engineering, Harbin Institute of Technology, Harbin, China.ORCID http://orcid.org/0000-0002-5969-1956
Xiaoshuai Huang *Biomedical Engineering Department, International Cancer Institute, Peking University Cancer Hospital and Institute, Health Science Center, Peking University, Beijing, China.
Jianyu Yang *The Key Laboratory of Weak-Light Nonlinear Photonics of Education Ministry, School of Physics and TEDA Institute of Applied Physics, Frontiers Science Center for Cell Responses, Nankai University, Tianjin, China.
Liying QuInnovation Photonics and Imaging Center, School of Instrumentation Science and Engineering, Harbin Institute of Technology, Harbin, China.
Guohua QiuState Key Laboratory of Membrane Biology, Beijing Key Laboratory of Cardiometabolic Molecular Medicine, Institute of Molecular Medicine, National Biomedical Imaging Center, School of Future Technology, Peking University, Beijing, China.
Yue ZhaoDepartment of Control Science and Engineering, Harbin Institute of Technology, Harbin, China.ORCID http://orcid.org/0000-0002-5970-5844
Xinwei WangInnovation Photonics and Imaging Center, School of Instrumentation Science and Engineering, Harbin Institute of Technology, Harbin, China.
Deer SuInnovation Photonics and Imaging Center, School of Instrumentation Science and Engineering, Harbin Institute of Technology, Harbin, China.
Xumin DingInnovation Photonics and Imaging Center, School of Instrumentation Science and Engineering, Harbin Institute of Technology, Harbin, China.ORCID http://orcid.org/0000-0002-7725-790X
Heng MaoSchool of Mathematical Sciences, Peking University, Beijing, China.
Yaming JiuUnit of Cell Biology and Imaging Study of Pathogen Host Interaction, The Center for Microbes, Development and Health, Key Laboratory of Molecular Virology and Immunology, Shanghai Institute of Immunity and Infection, Chinese Academy of Sciences, Shanghai, China.ORCID http://orcid.org/0000-0002-8601-8820
Ying HuSchool of Life Science and Technology, Harbin Institute of Technology, Harbin, China.
Jiubin TanKey Laboratory of Ultra-Precision Intelligent Instrumentation of Ministry of Industry and Information Technology, Harbin Institute of Technology, Harbin, China.
Shiqun ZhaoState Key Laboratory of Membrane Biology, Beijing Key Laboratory of Cardiometabolic Molecular Medicine, Institute of Molecular Medicine, National Biomedical Imaging Center, School of Future Technology, Peking University, Beijing, China. shiqunzhao@pku.edu.cn.
Leiting PanThe Key Laboratory of Weak-Light Nonlinear Photonics of Education Ministry, School of Physics and TEDA Institute of Applied Physics, Frontiers Science Center for Cell Responses, Nankai University, Tianjin, China. plt@nankai.edu.cn.ORCID http://orcid.org/0000-0001-5329-6114
Liangyi ChenState Key Laboratory of Membrane Biology, Beijing Key Laboratory of Cardiometabolic Molecular Medicine, Institute of Molecular Medicine, National Biomedical Imaging Center, School of Future Technology, Peking University, Beijing, China. lychen@pku.edu.cn.ORCID http://orcid.org/0000-0003-1270-7321
Haoyu LiInnovation Photonics and Imaging Center, School of Instrumentation Science and Engineering, Harbin Institute of Technology, Harbin, China. lihaoyu@hit.edu.cn.ORCID http://orcid.org/0000-0002-0004-9336

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In fluorescence microscopy, computational algorithms have been developed to suppress noise, enhance contrast, and even enable super-resolution (SR). However, the local quality of the images may vary on multiple scales, and these differences can lead to misconceptions. Current mapping methods fail to finely estimate the local quality, challenging to associate the SR scale content. Here, we develop a rolling Fourier ring correlation (rFRC) method to evaluate the reconstruction uncertainties down to SR scale. To visually pinpoint regions with low reliability, a filtered rFRC is combined with a modified resolution-scaled error map (RSM), offering a comprehensive and concise map for further examination. We demonstrate their performances on various SR imaging modalities, and the resulting quantitative maps enable better SR images integrated from different reconstructions. Overall, we expect that our framework can become a routinely used tool for biologists in assessing their image datasets in general and inspire further advances in the rapidly developing field of computational imaging.

Identifiers

PMID38097537
PMCPMC10721804

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.