Evidence map›Paper›PMID 38094626›Full record

ArticleFrontiers in microbiology2023

Impact of wet-lab protocols on quality of whole-genome short-read sequences from foodborne microbial pathogens.

Leonie F Forth, Erik Brinks, Grégoire Denay, Ahmad Fawzy, Stefan Fiedler, Jannika Fuchs, Anne-Catrin Geuthner, Thomas Hankeln, Ekkehard Hiller, Larissa Murr and 15 more

Abstract read
In one paragraph

Article in Frontiers in microbiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

25 authors.

Leonie F ForthDepartment of Biological Safety, German Federal Institute for Risk Assessment, Berlin, Germany.
Erik BrinksInstitute of Microbiology and Biotechnology, Max Rubner-Institut, Kiel, Germany.
Grégoire DenayChemical and Veterinary Analytical Institute Rhein-Ruhr-Wupper (CVUA-RRW), Krefeld, Germany.
Ahmad FawzyDepartment of Veterinary Medicine, Hessian State Laboratory, Giessen, Germany.
Stefan FiedlerMethod Standardisation, Reference Laboratories, Resistance to Antibiotics, Federal Office of Consumer Protection and Food Safety, Berlin, Germany.
Jannika FuchsChemical and Veterinary Analysis Agency Karlsruhe, Karlsruhe, Germany.
Anne-Catrin GeuthnerDepartment of Food Safety, State Office for Consumer Protection Saxony-Anhalt, Halle, Germany.
Thomas HankelnInstitute of Organismic and Molecular Evolution, Johannes Gutenberg University Mainz, Mainz, Germany.
Ekkehard HillerChemical and Veterinary Analysis Agency Stuttgart, Fellbach, Germany.
Larissa MurrBavarian Health and Food Safety Authority, Oberschleißheim, Germany.
Henning PetersenChemical and Veterinary Analytical Institute Ostwestfalen-Lippe, Detmold, Germany.
Ralf ReitingHessian State Laboratory, Kassel, Germany.
Christian SchäfersInstitute for Hygiene and Environment, Hamburg, Germany.
Claudia SchwabLabor Kneißler GmbH & Co. KG, Burglengenfeld, Germany.
Kathrin SzaboMethod Standardisation, Reference Laboratories, Resistance to Antibiotics, Federal Office of Consumer Protection and Food Safety, Berlin, Germany.
Andrea ThürmerMethods Development and Research Infrastructure, Robert Koch-Institut, Berlin, Germany.
Anne WöhlkeFood and Veterinary Institute Braunschweig/Hannover, Lower Saxony State Office for Consumer Protection and Food Safety, Braunschweig, Germany.
Jennie FischerDepartment of Biological Safety, German Federal Institute for Risk Assessment, Berlin, Germany.
Stefanie LüthDepartment of Biological Safety, German Federal Institute for Risk Assessment, Berlin, Germany.
Michaela ProjahnDepartment of Biological Safety, German Federal Institute for Risk Assessment, Berlin, Germany.
Kerstin StinglDepartment of Biological Safety, German Federal Institute for Risk Assessment, Berlin, Germany.
Maria BorowiakDepartment of Biological Safety, German Federal Institute for Risk Assessment, Berlin, Germany.
Carlus DenekeDepartment of Biological Safety, German Federal Institute for Risk Assessment, Berlin, Germany.
Burkhard MalornyDepartment of Biological Safety, German Federal Institute for Risk Assessment, Berlin, Germany.
Laura UelzeDepartment of Biological Safety, German Federal Institute for Risk Assessment, Berlin, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

For successful elucidation of a food-borne infection chain, the availability of high-quality sequencing data from suspected microbial contaminants is a prerequisite. Commonly, those investigations are a joint effort undertaken by different laboratories and institutes. To analyze the extent of variability introduced by differing wet-lab procedures on the quality of the sequence data we conducted an interlaboratory study, involving four bacterial pathogens, which account for the majority of food-related bacterial infections:

Indexed as

CampylobacterEscherichiainterlaboratory studyListeriaprotocolqualitySalmonellawhole genome sequencing

Identifiers

PMID38094626
PMCPMC10716212

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.