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ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2023

Identification and fine-mapping of QYrAS286-2BL conferring adult-plant resistance to stripe rust in cultivated emmer wheat.

Yuqin Li, Yanling Hu, Yun Jiang, Qiang Zhou, Yu He, Jingshu He, Xuejiao Chen, Xue Chen, Bo Jiang, Ming Hao and 9 more

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Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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19 authors.

Yuqin Li *State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Yanling Hu *Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Yun JiangBiotechnology and Nuclear Technology Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610061, Sichuan, China.
Qiang ZhouChengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, Sichuan, China.
Yu HeTriticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Jingshu HeTriticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Xuejiao ChenTriticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Xue ChenTriticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Bo JiangTriticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Ming HaoTriticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Shunzong NingTriticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Zhongwei YuanTriticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Jinrui ZhangTriticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Chongjing XiaWheat Research Institute, School of Life Sciences and Engineering, Southwest University of Science and Technology, Mianyang, 621010, Sichuan, China.
Bihua WuTriticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Lihua FengState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Lianquan ZhangState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. zhanglianquan1977@126.com.
Dengcai LiuState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. dcliu7@sicau.edu.cn.
Lin HuangTriticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. lhuang@sicau.edu.cn.ORCID http://orcid.org/0000-0003-4753-3683

Funding

National Natural Science Foundation of China 32272068,31801360
6 · The paper itself

Abstract

key messageA novel major adult-plant stripe rust resistance QTL derived from cultivated emmer wheat was mapped to a 123.6-kb region on wheat chromosome 2BL. Stripe rust, caused by the fungal pathogen Puccinia striiformis f. sp. tritici (Pst), is one of the most devastating diseases of wheat. Identification of new sources of resistance and their utilization in breeding programs is the effectively control strategy. The objective of this study was to identify and genetically characterize the stripe rust resistance derived from the cultivated emmer accession AS286. A recombinant inbred line population, developed from a cross between the susceptible durum wheat line langdon and AS286, was genotyped using the Wheat55K single nucleotide polymorphism array and evaluated in field conditions with a mixture of the prevalent Chinese Pst races (CYR32, CYR33, CYR34, Zhong4, and HY46) and in growth chamber with race CYR34. Three QTLs conferring resistance were mapped on chromosomes 1BS, 2BL, and 5BL, respectively. The QYrAS286-1BS and QYrAS286-2BL were stable with major effects, explaining 12.91% to 18.82% and 11.31% to 31.43% of phenotypic variation, respectively. QYrAS286-5BL was only detected based on growth chamber seedling data. RILs harboring both QYrAS286-1BS and QYrAS286-2BL showed high levels of stripe rust resistance equal to the parent AS286. The QYrAS286-2BL was only detected at the adult-plant stage, which is different from previously named Yr genes and inherited as a single gene. It was further mapped to a 123.6-kb region using KASP markers derived from SNPs identified by bulked segregant RNA sequencing (BSR-Seq). The identified loci enrich our stripe rust resistance gene pool, and the flanking markers developed here could be useful in marker-assisted selection for incorporating QYrAS286-2BL into wheat cultivars.

Indexed as

BasidiomycotaTriticumChromosome MappingDisease ResistanceGenotypePlant BreedingPlant DiseasesQuantitative Trait Loci

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.