Evidence map›Paper›PMID 38089797›Full record

ArticleFrontiers in plant science2023

Selection and validation of reference genes for normalizing qRT‒PCR gene expression studies in

Imran Ul Haq, Siddra Ijaz, Abeer Hashem, Graciela Dolores Avila-Quezada, Elsayed Fathi Abd Allah

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in plant science, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 3 countries.

Imran Ul HaqDepartment of Plant Pathology, University of Agriculture Faisalabad, Faisalabad, Pakistan.
Siddra IjazCentre of Agricultural Biochemistry and Biotechnology, University of Agriculture Faisalabad, Faisalabad, Pakistan.
Abeer HashemBotany and Microbiology Department, College of Science, King Saud University, Riyadh, Saudi Arabia.
Graciela Dolores Avila-QuezadaFacultad de Ciencias Agrotecnológicas, Universidad Autónoma de Chihuahua, Chihuahua, Chihuahua, Mexico.
Elsayed Fathi Abd AllahPlant Production Department, College of Food and Agricultural Sciences, King Saud University, Riyadh, Saudi Arabia.
King Saud University · SAUniversity of Agriculture Faisalabad · PKAutonomous University of Chihuahua · MX

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Quantitative real-time PCR is used to quantify gene expression, even to detect low-level transcripts. It detects and quantifies the inoculum level of fungal pathogens in infected hosts. However, reliable expression profiling data require accurate transcript normalization against a stable reference gene. Hence, using stably expressed reference genes under variable conditions is paramount in gene expression analysis. In the current study, reference genes were selected and validated in

Indexed as

Colletotrichum gloeosporioidespathogenesis-related genesPsidium guajavaqRT−PCRreference genes

Identifiers

PMID38089797
PMCPMC10712560
OpenAlexW4388942506

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.