Evidence map›Paper›PMID 38088106›Full record

ArticleExperimental biology and medicine (Maywood, N.J.)2023

Improved protocol for

Lucas Rodrigo de Souza, Italo Esposti Poly da Silva, Gabriele Celis-Silva, Bruna Winkert Raddatz, Louise Matiê Imamura, Edson Yu Sin Kim, Gabriel Vieira Valderrama, Halanna de Paula Riedi, Sergio Renato Rogal, Bernardo Montesanti Machado de Almeida and 3 more

Open access · greenAbstract read
In one paragraph

Article in Experimental biology and medicine (Maywood, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.7field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 1 institution in 1 country.

Lucas Rodrigo de SouzaCenter for Molecular Biology and Genetic Engineering (CBMEG), Universidade Estadual de Campinas (UNICAMP), Campinas/SP 13083-875, Brazil.
Italo Esposti Poly da SilvaCenter for Molecular Biology and Genetic Engineering (CBMEG), Universidade Estadual de Campinas (UNICAMP), Campinas/SP 13083-875, Brazil.
Gabriele Celis-SilvaCenter for Molecular Biology and Genetic Engineering (CBMEG), Universidade Estadual de Campinas (UNICAMP), Campinas/SP 13083-875, Brazil.
Bruna Winkert RaddatzHilab, Curitiba/PR 81270-185, Brazil.
Louise Matiê ImamuraHilab, Curitiba/PR 81270-185, Brazil.ORCID 0000-0001-8234-6010
Edson Yu Sin KimHilab, Curitiba/PR 81270-185, Brazil.
Gabriel Vieira ValderramaCenter for Molecular Biology and Genetic Engineering (CBMEG), Universidade Estadual de Campinas (UNICAMP), Campinas/SP 13083-875, Brazil.
Halanna de Paula RiediHilab, Curitiba/PR 81270-185, Brazil.
Sergio Renato RogalHilab, Curitiba/PR 81270-185, Brazil.
Bernardo Montesanti Machado de AlmeidaHilab, Curitiba/PR 81270-185, Brazil.
Marcus Vinícius Mazega FigueredoHilab, Curitiba/PR 81270-185, Brazil.
Mario Henrique BengtsonCenter for Medicinal Chemistry (CQMED), Universidade Estadual de Campinas (UNICAMP), Campinas/SP 13083-886, Brazil.
Katlin Brauer MassirerCenter for Molecular Biology and Genetic Engineering (CBMEG), Universidade Estadual de Campinas (UNICAMP), Campinas/SP 13083-875, Brazil.ORCID 0000-0001-6390-2560
Universidade Estadual de Campinas (UNICAMP) · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has raised awareness in the scientific community about the importance of being prepared for sanitary emergencies. Many measures implemented during the COVID pandemic are now being expanded to other applications. In the field of molecular and immunological diagnostics, the need to massively test the population worldwide resulted in the application of a variety of methods to detect viral infection. Besides gold standard reverse transcription quantitative polymerase chain reaction (RT-qPCR), the use of reverse transcription loop-mediated isothermal amplification (RT-LAMP) arose as an alternative and sensitive method to amplify and detect viral genetic material. We have used openly available protocols and have improved the protein production of RT-LAMP enzymes

Indexed as

COVID-19COVID-19 TestingHumansMolecular Diagnostic TechniquesPoint-of-Care TestingReverse Transcriptase Polymerase Chain ReactionRNA-Directed DNA PolymeraseSARS-CoV-2Sensitivity and SpecificityRNA-Directed DNA PolymeraseBst polymerasepoint-of-carereverse transcriptaseRNA virus detectionRT-LAMPSARS-CoV-2

Identifiers

PMID38088106
PMCPMC10723028
OpenAlexW4389680516

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.