Evidence map›Paper›PMID 38084978›Full record

ArticleMicrobiology spectrum2024

Evaluation of ready-to-use freezer stocks of a synthetic microbial community for maize root colonization.

J Jacob Parnell, Simina Vintila, Clara Tang, Maggie R Wagner, Manuel Kleiner

Abstract read
In one paragraph

Article in Microbiology spectrum, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Article
  2. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

J Jacob Parnell *Department of Plant and Microbial Biology, North Carolina State University , Raleigh, North Carolina, USA.
Simina Vintila *Department of Plant and Microbial Biology, North Carolina State University , Raleigh, North Carolina, USA.
Clara TangDepartment of Plant and Microbial Biology, North Carolina State University , Raleigh, North Carolina, USA.
Maggie R WagnerDepartment of Ecology and Evolutionary Biology, University of Kansas , Lawrence, Kansas, USA.
Manuel KleinerDepartment of Plant and Microbial Biology, North Carolina State University , Raleigh, North Carolina, USA.ORCID 0000-0001-6904-0287

Funding

National Science Foundation (NSF) 2033621Novo Nordisk Fonden (NNF) NNF19SA0059360U.S. Department of Agriculture (USDA) 2022-67013-36672
6 · The paper itself

Abstract

importanceSynthetic communities (SynComs) are an invaluable tool to characterize and model plant-microbe interactions. Multimember SynComs approximate intricate real-world interactions between plants and their microbiome, but the complexity and time required for their construction increase enormously for each additional member added to the SynCom. Therefore, researchers who study a diversity of microbiomes using SynComs are looking for ways to simplify the use of SynComs. In this manuscript, we evaluate the feasibility of creating ready-to-use freezer stocks of a well-studied seven-member SynCom for maize roots. The frozen ready-to-use SynCom stocks work according to the principle of "just add buffer and apply to sterilized seeds or seedlings" and thus can save time applied in multiple days of laborious growing and combining of multiple microorganisms. We show that ready-to-use SynCom stocks provide comparable results to those of freshly constructed SynComs and thus allow for significant time savings when working with SynComs.

Indexed as

MicrobiotaZea maysBacteriaPlant RootsPlantsSoil Microbiologymaizeplant–microbe interactionsplant microbiomeroot colonizationSynComSynthetic communities

Identifiers

PMID38084978
PMCPMC10783020

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.