ArticleMicrobiome2023
Application of culturomics in fungal isolation from mangrove sediments.
Article in Microbiome, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers, 1 of them a synthesis that pooled it.
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Who cites it
32 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Worldwide diversity and ecology of mangrove fungi: a systematic review of ITS metabarcoding studies and a quantitative, integrative analysis of raw sequence data.World journal of microbiology & biotechnology · 2026Pooled it
- Precipitation reduction mitigates the negative impact of nitrogen deposition on soil nitrogen fixation in subtropical Chinese fir plantations.Science China. Life sciences · 2026Article
- Unlocking Microbial Dark Matter: A Comprehensive Review of Isolation Technologies from Traditional Culturing to Single-Cell Technologies.Microorganisms · 2026Review
- New insights into acremonium-like fungi inStudies in mycology · 2026Article
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- High-throughput single-cell isolation ofMicrobiology spectrum · 2026Article
- Antimicrobial potential of bacteria isolated from sediment andFrontiers in microbiology · 2026Article
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- Metabarcoding insights into the fungal diversity and biotechnological potential of mangrove sediments in Ecuador's Reserva Ecológica Manglares Churute.Frontiers in fungal biology · 2026Article
- Biogeographic patterns, assembly processes, and functional potential of root-associated microbiomes across the native range of the endangered orchidFrontiers in microbiology · 2026Article
- Taxonomy and molecular evaluation of the Halosphaeriaceae (Microascales, Hypocreomycetidae).MycoKeys · 2026Article
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- Mining Microbial Dark Matter: Advanced Cultivation Techniques for Bioactive Compound Discovery.Pharmaceuticals (Basel, Switzerland) · 2025Review
- Seven New Series and Four New Species in SectionsJournal of fungi (Basel, Switzerland) · 2025Article
- Five New Hypocrealean Species from Algae and Sediment in the Intertidal Zones of China.Journal of fungi (Basel, Switzerland) · 2025Article
- A novel, exclusively lichen-inhabiting lineage of hypocrealean fungi revealed in thePersoonia · 2025Article
- Article
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Authors and funding
9 authors.
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Abstract
backgroundFungi play a crucial role in ecosystems, and they have been widely considered a promising source for natural compounds that are crucial for drug discovery. Fungi have a high diversity, but about 95% of them remain unknown to science. The description rate of fungi is very low, mainly due to the inability of most fungi to grow in artificial media, which could not provide a sufficiently similar environment to their natural habitats. Moreover, many species in nature are in a state of low metabolic activity which cannot readily proliferate without proper resuscitation. Previously developed culturomics techniques are mostly designed and applicable for bacteria, with few attempts for fungal isolation because of their significantly larger cell size and hyphal growth properties.
resultsThis study attempted to isolate previously uncultured and rare fungi from mangrove sediments using newly developed fungal enrichment culture method (FECM) and fungal isolation chips (FiChips). Comparison of fungal community composition at different enrichment stages showed that FECM had great influence on fungal community composition, with rare taxa increased significantly, thus improving the isolation efficiency of previously uncultured fungi. Similarly, in situ cultivation using FiChips has a significant advantage in detecting and culturing rare fungi, as compared to the conventional dilution plate method (DPM). In addition, based on morphological comparisons and phylogenetic analyses, we described and proposed 38 new ascomycetous taxa, including three new families, eight new genera, 25 new species, and two new combinations (presented in additional file 1).
conclusionsOur study demonstrated that mangrove sediments harbor a high diversity of fungi, and our new isolation approaches (FECM and FiChips) presented a high efficiency in isolating hitherto uncultured fungi, which is potentially usable for fungal isolation in other similar environments. Video Abstract.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.