Evidence map›Paper›PMID 38082138›Full record

ArticleArchives of virology2023

Differential role of NSs genes in the neurovirulence of two genogroups of Akabane virus causing postnatal encephalomyelitis.

Misuzu Okajima, Akiko Takenaka-Uema, Yuji Fujii, Fumiki Izumi, Isshu Kojima, Makoto Ozawa, Kiyotada Naitou, Yuto Suda, Shoko Nishiyama, Shin Murakami and 5 more

Abstract read
PubMed Publisher
In one paragraph

Article in Archives of virology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.2field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 4 institutions in 1 country.

Misuzu OkajimaJoint Graduate School of Veterinary Sciences, Gifu University, Gifu, Japan.
Akiko Takenaka-UemaLaboratory of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Yuji FujiiJoint Graduate School of Veterinary Sciences, Gifu University, Gifu, Japan.
Fumiki IzumiJoint Graduate School of Veterinary Sciences, Gifu University, Gifu, Japan.
Isshu KojimaJoint Graduate School of Veterinary Medicine, Kagoshima University, Kagoshima, Japan.
Makoto OzawaJoint Graduate School of Veterinary Medicine, Kagoshima University, Kagoshima, Japan.
Kiyotada NaitouDepartment of Basic Veterinary Science, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima, Japan.
Yuto SudaKagoshima Research Station, National Institute of Animal Health, NARO, Kagoshima, Japan.
Shoko NishiyamaLaboratory of Zoonotic Diseases, Faculty of Applied Biological Sciences, Gifu University, Gifu, Japan.
Shin MurakamiLaboratory of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Taisuke HorimotoLaboratory of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Naoto ItoJoint Graduate School of Veterinary Sciences, Gifu University, Gifu, Japan.
Hiroaki ShirafujiKagoshima Research Station, National Institute of Animal Health, NARO, Kagoshima, Japan.
Tohru YanaseKagoshima Research Station, National Institute of Animal Health, NARO, Kagoshima, Japan.
Tatsunori MasataniJoint Graduate School of Veterinary Sciences, Gifu University, Gifu, Japan. masatani.tatsunori.j9@f.gifu-u.ac.jp.ORCID http://orcid.org/0000-0002-2347-4079
Gifu University · JPKagoshima University · JPNational Institute of Animal Health · JPThe University of Tokyo · JP

Funding

Japan Science and Technology Agency JPMJSP2125Japan Society for the Promotion of Science 21K05924
6 · The paper itself

Abstract

Akabane virus (AKAV) is a member of the genus Orthobunyavirus, family Peribunyaviridae. In addition to AKAV strains that cause fetal Akabane disease, which is characterized by abortion in ruminants, some AKAV strains cause postnatal infection characterized by nonsuppurative encephalomyelitis in ruminants. Here, we focused on the NSs protein, a virulence factor for most viruses belonging to the genus Orthobunyavirus, and we hypothesized that this protein would act as a neurovirulence factor in AKAV strains causing postnatal encephalomyelitis. We generated AKAV strains that were unable to produce the NSs protein, derived from two different genogroups, genogroups I and II, and then examined the role of their NSs proteins by inoculating mice intracerebrally with these modified viruses. Our results revealed that the neurovirulence of genogroup II strains is dependent on the NSs protein, whereas that of genogroup I strains is independent of this protein. Notably, infection of primary cultured bovine cells with these viruses suggested that the NSs proteins of both genogroups suppress innate immune-related gene expression with equal efficiency. These results indicate differences in the determinants of virulence of orthobunyaviruses.

Indexed as

Bunyaviridae InfectionsEncephalomyelitisOrthobunyavirusAnimalsCattleFemaleGenotypeMicePregnancyRuminants

Identifiers

PMID38082138
OpenAlexW4389545944

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.