Evidence map›Paper›PMID 38081607›Full record

ArticleNan fang yi ke da xue xue bao = Journal of Southern Medical University2023

[Oridonin suppresses the effect of thioacetamide for promoting osteoclast differentiation of RAW264.7 cells and inhibiting osteoblast differentiation of bone mesenchymal stem cells].

X Jin, J Xu, X Chen, J Chen, H Huang, T Zhang, J Ren, J Xu

Open access · greenAbstract readEnglish Abstract
PubMed Full text at PMC Publisher
In one paragraph

Article in Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

X JinSchool of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou 310053, China.
J XuSchool of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou 310053, China.
X ChenSchool of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou 310053, China.
J ChenSchool of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou 310053, China.
H HuangSchool of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou 310053, China.
T ZhangSchool of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou 310053, China.
J RenSchool of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou 310053, China.
J XuSchool of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou 310053, China.
Zhejiang Chinese Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo explore the effect of oridonin (ORI) for suppressing thioacetamide (TAA)-induced osteoclast differentiation of RAW264.7 cells and antagonizing the inhibitory effect of TAA on osteogenic differentiation of bone mesenchymal stem cells (BMSCs).

methodsThe effects of TAA and ORI on the proliferation of RAW264.7 cells and SD rat BMSCs were examined using CCK-8 assay. TRAP staining and immunofluorescence staining were used to observe the effects of TAA and ORI on osteoclast differentiation in RAW264.7 cells. The expressions of osteoclast-specific proteins in the treated cells were detected using Western blotting, and p65 nuclear translocation and reactive oxygen species (ROS) production in the cells were assessed with immunofluorescence assay and flow cytometry. Alkaline phosphatase (ALP) staining and alizarin red staining were used to examine the effects of TAA and ORI on osteogenic differentiation of BMSCs, and the expressions of osteogenic and apoptosis-related proteins in the cells were detected with Western blotting.

resultsCompared with RAW264.7 cells treated with TAA alone, the cells with the combined treatment with TAA and ORI showed decreased osteoclast differentiation (

conclusionORI inhibits TAA-induced osteoclast differentiation

Indexed as

Mesenchymal Stem CellsOsteoporosisAnimalsCell DifferentiationCells, CulturedCore Binding Factor Alpha 1 SubunitDiterpenes, KauraneNF-kappa BOsteoblastsOsteoclastsOsteogenesisRatsRats, Sprague-DawleyReactive Oxygen SpeciesThioacetamideCore Binding Factor Alpha 1 SubunitDiterpenes, KauraneNF-kappa BoridoninReactive Oxygen SpeciesThioacetamideoridoninosteoclastogenesisosteogenesisosteoporosisthioacetamide

Identifiers

PMID38081607
PMCPMC10713462
OpenAlexW4389801316

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.