Evidence map›Paper›PMID 38077446›Full record

ArticlePeerJ2023

Genomic DNA extraction optimization and validation for genome sequencing using the marine gastropod Kellet's whelk.

Benjamin N Daniels, Jenna Nurge, Olivia Sleeper, Andy Lee, Cataixa López, Mark R Christie, Robert J Toonen, Crow White, Jean M Davidson

Open access · goldAbstract read
In one paragraph

Article in PeerJ, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.8field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 9 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Benjamin N DanielsCenter for Coastal Marine Sciences, California Polytechnic State University - San Luis Obispo, San Luis Obispo, CA, United States of America.
Jenna NurgeBiological Sciences Department, California Polytechnic State University - San Luis Obispo, San Luis Obispo, CA, United States of America.
Olivia SleeperBiological Sciences Department, California Polytechnic State University - San Luis Obispo, San Luis Obispo, CA, United States of America.
Andy LeeDepartment of Biological Sciences, Purdue University, West Lafayette, IN, United States of America.
Cataixa LópezHawai'i Institute of Marine Biology, University of Hawai'i at Mānoa, Kāne'ohe, HI, United States of America.
Mark R ChristieDepartment of Biological Sciences, Purdue University, West Lafayette, IN, United States of America.
Robert J ToonenHawai'i Institute of Marine Biology, University of Hawai'i at Mānoa, Kāne'ohe, HI, United States of America.
Crow WhiteCenter for Coastal Marine Sciences, California Polytechnic State University - San Luis Obispo, San Luis Obispo, CA, United States of America.
Jean M DavidsonBiological Sciences Department, California Polytechnic State University - San Luis Obispo, San Luis Obispo, CA, United States of America.
California Polytechnic State University · USPurdue University West Lafayette · USUniversity of Hawaiʻi at Mānoa · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Next-generation sequencing technologies, such as Nanopore MinION, Illumina Hiseq and Novaseq, and PacBio Sequel II, hold immense potential for advancing genomic research on non-model organisms, including the vast majority of marine species. However, application of these technologies to marine invertebrate species is often impeded by challenges in extracting and purifying their genomic DNA due to high polysaccharide content and other secondary metabolites. In this study, we help resolve this issue by developing and testing DNA extraction protocols for Kellet's whelk (

Indexed as

GastropodaAnimalsDNAGenomeGenomicsSequence Analysis, DNADNADNA extractionGeneticsGenome sequencingPopulation geneticsProtocol optimization

Identifiers

PMID38077446
PMCPMC10710129
OpenAlexW4389397906

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.