ArticleNeurophotonics2023
Evaluation of compact pulsed lasers for two-photon microscopy using a simple method for measuring two-photon excitation efficiency.
Article in Neurophotonics, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
6 citing papers in PubMed, 7 citations in OpenAlex.
- Vector characteristics of sub-ps 1.9 µm pulses produced by non-PM thulium-doped all-fiber MOPA.Scientific reports · 2026Article
- Standardized measurements for monitoring and comparing multiphoton microscope systems.Nature protocols · 2025Review
- TWINKLE: An open-source two-photon microscope for teaching and research.bioRxiv : the preprint server for biology · 2025Article
- Article
- Special Section Guest Editorial: Open-source neurophotonic tools for neuroscience.Neurophotonics · 2024Article
- Standardised Measurements for Monitoring and Comparing Multiphoton Microscope Systems.bioRxiv : the preprint server for biology · 2024Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Significance: Two-photon (2p) microscopy has historically relied on titanium sapphire pulsed lasers that are expensive and have a large footprint. Recently, several manufacturers have developed less expensive compact pulsed lasers optimized for 2p excitation of green fluorophores. However, quantitative evaluation of their quality is lacking. Aim: We describe a simple approach to systematically evaluate 2p excitation efficiency, an empiric measure of the quality of a pulsed laser and its ability to elicit 2p induced fluorescence. Approach: By measuring pulse width, repetition rate, and fluorescence output, we calculated a measure of 2p excitation efficiency Results: 2p excitation efficiency varied substantially among tested lasers. The Coherent Axon exhibited the best 2p excitation efficiency ( Conclusions: We describe a simple method that both laser developers and end users can use to benchmark the 2p excitation efficiency of lasers used for 2p microscopy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.