ArticleBiochemistry and biophysics reports2023
Guidelines for an optimized differential centrifugation of cells.
Article in Biochemistry and biophysics reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it.
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- Utilizing an In Vitro Fermentation Model to Assess Probiotics onAnimals : an open access journal from MDPI · 2026Article
- Rapid Detection of Tetracycline Residues in Milk Using Colorimetric Sensor Based on Polymethylmethacrylate (PMMA) and Polystyrene (PS) Polymers.Journal of analytical methods in chemistry · 2026Article
- Bioprocess Engineering for Cultivated Meat.Advances in biochemical engineering/biotechnology · 2026Review
- Advances and Applications of Spatial Proteomics: From Organellar Maps to Clinical Translation.Chembiochem : a European journal of chemical biology · 2026Review
- Standardizing Bacterial Extracellular Vesicle Purification: A Call for Consensus.Journal of microbiology and biotechnology · 2025Review
- Innovative Method for Fully Automated, Enzyme-Free Tissue Dissociation and Preparation for Single-Cell Analysis.Cellular and molecular bioengineering · 2025Article
- Laboratory preparation methods for human-derived fecal microbial suspensions for fecal microbiota transplantation: a review and standardization perspectives.Frontiers in microbiology · 2025Review
- Development of an easy non-destructive particle isolation protocol for quality control of red blood cell concentrates.Journal of extracellular biology · 2025Article
- Cancer stem cells in hepatocellular carcinoma: therapy resistance and emerging treatments.Frontiers in immunology · 2025Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
Literature reviews reveal a significant deficiency in conceptual comprehension concerning centrifugation, a crucial step in both medical and research protocols. The arbitrary fluctuations in centrifugal forces present a potential threat to the reproducibility of results. To address this, we propose concise guidelines that integrate key factors such as temperature, osmolarity, fluid volume, and viscosity. These guidelines aim to enhance comprehension of optimal sedimentation conditions for cell suspensions. Additionally, we introduce a standardized protocol for determining the optimal RCF and centrifugation time. The goal is to maximize sedimentation efficiency while minimizing cell damage, contributing to a universally applicable and reproducible method in centrifugation practices.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.