Evidence map›Paper›PMID 38073881›Full record

ArticleInfectious medicine2023

A study implementing real-time PCR to identify

Le Duc Vinh, Nguyen Kim Thach, Huynh Hong Quang, Do Nhu Binh, Tran Thi Duc Hanh, Nguyen Minh Toàn, Nguyen Trung Tuyen, Nguyen Thu Huong

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Infectious medicine, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Le Duc VinhDepartment of Medical Parasitology, Pham Ngoc Thach University of Medicine, Ho Chi Minh City 700000, Vietnam.
Nguyen Kim ThachDepartment of Fundamental Chemistry-Biochemistry, Pham Ngoc Thach University of Medicine, Ho Chi Minh City 700000, Vietnam.
Huynh Hong QuangClinical Epidemiology Research Unit, Institute of Malariology, Parasitology, and Entomology Quy Nhon, Binh Dinh 55000, Vietnam.
Do Nhu BinhDepartment of Infectious Disease, Military Hospital 103, Hanoi 100000, Vietnam.
Tran Thi Duc HanhDepartment of Epidemiology, Hanoi University of Public Health, Hanoi 100000, Vietnam.
Nguyen Minh ToànDepartment of Microbiology - Parasitology, Hanoi University of Public Health, Hanoi 100000, Vietnam.
Nguyen Trung TuyenDepartment of Medicine, University of Medicine and Pharmacy, Vietnam National University, Hanoi, 100000 Vietnam.
Nguyen Thu HuongDepartment of Microbiology - Parasitology, Hanoi University of Public Health, Hanoi 100000, Vietnam.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Strongyloidiasis, a neglected disease caused by intestinal nematodes of the genus, is endemic to tropical and subtropical areas such as Vietnam. Morphological methods only identify the genus, while DNA-molecular techniques are susceptible in Methods: A cross-sectional study used 1190 stool specimens collected from July 2017 to November 2018. All samples were transported within 2 h, stored at 2-8°C, and processed within 48 h for microscopy smear and culture at the Laboratory of Medical Parasitology, Pham Ngoc Thach University of Medicine (PNT). Then all positive samples with the above 2 methods were verified by real-time PCR technique. Real-time PCR amplification was conducted at the Laboratory of Molecular Biology, PNT. Results: Direct microscopy and modified Harada-Mori culture detected Conclusion: DNA-molecular techniques and sequence analysis are highly suitable for identifying

Indexed as

DNA-molecular techniquesStrongyloides rattiStrongyloides stercoralisStrongyloidiasis

Identifiers

PMID38073881
PMCPMC10699675

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.