Evidence map›Paper›PMID 38073442›Full record

ArticleCritical reviews in eukaryotic gene expression2024

Bromodomain Proteins Epigenetically Regulate the Mitotically Associated lncRNA MANCR in Triple Negative Breast Cancer Cells.

Kirsten M Tracy, Shannon Prior, Willem T Trowbridge, Joseph R Boyd, Prachi N Ghule, Seth Frietze, Janet L Stein, Gary S Stein, Jane B Lian

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Critical reviews in eukaryotic gene expression, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.5field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. The role of super-enhancer-driven lncRNAs in cancer.Computational and structural biotechnology journal · 2025
    Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Kirsten M TracyDepartment of Biochemistry, University of Vermont Larner College of Medicine, Burlington, VT 05405.
Shannon PriorDepartment of Biomedical and Health Sciences, University of Vermont College of Nursing and Health Sciences, Burlington, VT 05405.
Willem T TrowbridgeDepartment of Biochemistry, University of Vermont Larner College of Medicine, Burlington, VT 05405.
Joseph R BoydDepartment of Biomedical and Health Sciences, University of Vermont College of Nursing and Health Sciences, Burlington, VT 05405.
Prachi N GhuleDepartment of Biochemistry, University of Vermont Larner College of Medicine, Burlington, VT 05405; Department of Biomedical and Health Sciences, University of Vermont College of Nursing and Health Sciences, Burlington, VT 05405.
Seth FrietzeDepartment of Biochemistry, University of Vermont Larner College of Medicine, Burlington, VT 05405; Department of Biomedical and Health Sciences, University of Vermont College of Nursing and Health Sciences, Burlington, VT 05405; University of Vermont Cancer Center, University of Vermont Larner College of Medicine, Burlington, VT 05405.
Janet L SteinDepartment of Biochemistry, University of Vermont Larner College of Medicine, Burlington, VT 05405; University of Vermont Cancer Center, University of Vermont Larner College of Medicine, Burlington, VT 05405.
Gary S SteinDepartment of Biochemistry, University of Vermont Larner College of Medicine, Burlington, VT 05405; University of Vermont Cancer Center, University of Vermont Larner College of Medicine, Burlington, VT 05405.
Jane B LianDepartment of Biochemistry, University of Vermont Larner College of Medicine, Burlington, VT 05405; University of Vermont Cancer Center, University of Vermont Larner College of Medicine, Burlington, VT 05405.
University of Vermont · US

Funding

SUBNUCLEAR TARGETING OF TRANSCRIPTION FACTORSP01CA082834 · NCI · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI LIAN, JANE B. · 2001 to 2015
$18.4M
Project 3: MANCR Mediates Epigenetic Mechanisms for Survival of Advanced Breast CancerP01CA240685 · NCI · UNIVERSITY OF VERMONT & ST AGRIC COLLEGE · PI STEIN, JANET L · 2021 to 2025
$8.7M
Chromatin Organization Regulates OsteogenesisR01DE029311 · NIDCR · UNIVERSITY OF VERMONT & ST AGRIC COLLEGE · PI LIAN, JANE B., STEIN, GARY S. · 2020 to 2024
$2.5M
Supplement to AWD000033276 - Tracy fellowship - childcare costsF32CA236125 · NCI · UNIVERSITY OF VERMONT & ST AGRIC COLLEGE · PI TRACY, KIRSTEN · 2019 to 2022
$229k
NCI NIH HHS F32 CA236125NCI NIH HHS P01 CA082834NCI NIH HHS P01 CA240685NIDCR NIH HHS R01 DE029311
6 · The paper itself

Abstract

Long non-coding RNA (lncRNA)-mediated control of gene expression contributes to regulation of biological processes that include proliferation and phenotype, as well as compromised expression of genes that are functionally linked to cancer initiation and tumor progression. lncRNAs have emerged as novel targets and biomarkers in breast cancer. We have shown that mitotically associated lncRNA MANCR is expressed in triple-negative breast cancer (TNBC) cells and that it serves a critical role in promoting genome stability and survival in aggressive breast cancer cells. Using an siRNA strategy, we selectively depleted BRD2, BRD3, and BRD4, singly and in combination, to establish which bromodomain proteins regulate MANCR expression in TNBC cells. Our findings were confirmed by using in situ hybridization combined with immunofluorescence analysis that revealed BRD4, either alone or with BRD2 and BRD3, can support MANCR regulation of TNBC cells. Here we provide evidence for MANCR-responsive epigenetic control of super enhancers by histone modifications that are required for gene transcription to support cell survival and expression of the epithelial tumor phenotype in triple negative breast cancer cells.

Indexed as

RNA, Long NoncodingTriple Negative Breast NeoplasmsBromodomain Containing ProteinsCell Cycle ProteinsCell Line, TumorCell ProliferationCell SurvivalGene Expression Regulation, NeoplasticHumansNuclear ProteinsTranscription FactorsBRD4 protein, humanBromodomain Containing ProteinsCell Cycle ProteinsNuclear ProteinsRNA, Long NoncodingTranscription Factors

Identifiers

PMID38073442
PMCPMC11023627
OpenAlexW4386363364

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.