Evidence map›Paper›PMID 38071657›Full record

ArticleMarine biotechnology (New York, N.Y.)2023

Nuclear Transformation of the Marine Pennate Diatom Nitzschia sp. Strain NIES-4635 by Multi-Pulse Electroporation.

Koki Okada, Yu Morimoto, Yukine Shiraishi, Takashi Tamura, Shigeki Mayama, Takashi Kadono, Masao Adachi, Kentaro Ifuku, Michiko Nemoto

Abstract read
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In one paragraph

Article in Marine biotechnology (New York, N.Y.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Genetic engineering in diatoms: advances and prospects.The Plant journal : for cell and molecular biology · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Koki OkadaGraduate School of Environmental and Life Science, Okayama University, Okayama, 700-8530, Japan.
Yu MorimotoGraduate School of Environmental and Life Science, Okayama University, Okayama, 700-8530, Japan.
Yukine ShiraishiGraduate School of Environmental and Life Science, Okayama University, Okayama, 700-8530, Japan.
Takashi TamuraFaculty of Environmental, Life, Natural Science and Technology, Okayama University, Okayama, 700-8530, Japan.
Shigeki MayamaThe Advanced Support Center for Science Teachers, Tokyo Gakugei University, Tokyo, 184-8511, Japan.
Takashi KadonoFaculty of Agriculture and Marine Science, Kochi University, Otsu-200, Monobe, Nankoku, Kochi, 783-8502, Japan.
Masao AdachiFaculty of Agriculture and Marine Science, Kochi University, Otsu-200, Monobe, Nankoku, Kochi, 783-8502, Japan.
Kentaro IfukuGraduate School of Agriculture, Kyoto University, Kitashirakawa-oiwake, Sakyo, Kyoto, 606-8502, Japan.
Michiko NemotoFaculty of Environmental, Life, Natural Science and Technology, Okayama University, Okayama, 700-8530, Japan. mnemoto@okayama-u.ac.jp.

Funding

Japan Society for the Promotion of Science 18K05818
6 · The paper itself

Abstract

Nitzschia is one of the largest genera of diatoms found in a range of aquatic environments, from freshwater to seawater. This genus contains evolutionarily and ecologically unique species, such as those that have lost photosynthetic capacity or those that live symbiotically in dinoflagellates. Several Nitzschia species have been used as indicators of water pollution. Recently, Nitzschia species have attracted considerable attention in the field of biotechnology. In this study, a transformation method for the marine pennate diatom Nitzschia sp. strain NIES-4635, isolated from the coastal Seto Inland Sea, was established. Plasmids containing the promoter/terminator of the fucoxanthin chlorophyll a/c binding protein gene (fcp, or Lhcf) derived from Nitzschia palea were constructed and introduced into cells by multi-pulse electroporation, resulting in 500 μg/mL nourseothricin-resistant transformants with transformation frequencies of up to 365 colonies per 10

Indexed as

DiatomsStreptothricinsChlorophyll AElectroporationPlasmidsChlorophyll AStreptothricinsDiatomGenetic transformationMulti-pulse electroporationNitzschia

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.