ArticleNature communications2023
Total wash elimination for solid phase peptide synthesis.
Article in Nature communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed.
- Peptide-Enabled Nanoplatforms for Malaria and Leishmaniasis: From Intracellular Targeting to Translational Diagnostic Perspectives.ChemMedChem · 2026Review
- Marine Antimicrobial Peptides: From Ocean Biodiversity to Genome Mining, Multi-Omics Discovery, and Biotechnological Innovation in the Battle Against Antimicrobial Resistance.Probiotics and antimicrobial proteins · 2026Review
- Sustainable Fragment Peptide Synthesis (SFPS): Leveraging Oxyma as a Dual Resin Cleavage-Peptide Coupling Agent.ACS omega · 2026Article
- Multifactorial Perspective for Greening Solid-Phase Peptide Synthesis: Rigid Polyacrylate Macroporous Resin in Combination with Green Solvents.ChemSusChem · 2026Article
- Silica-Assisted Solid-Phase Peptide Synthesis (SiPPS).Journal of peptide science : an official publication of the European Peptide Society · 2025Article
- Tetrahydropyranyl Backbone Protection for Enhanced Fmoc Solid-Phase Peptide Synthesis.Chemistry (Weinheim an der Bergstrasse, Germany) · 2025Article
- Backbone Protecting Groups for Enhanced Peptide and Protein Synthesis.Angewandte Chemie (International ed. in English) · 2025Review
- Toward a Green SPPS: The Use of an Innovative Mesoporous pDVB Support for Environmentally Friendly Solvents.Journal of peptide science : an official publication of the European Peptide Society · 2025Article
- On-Resin Recycling of Acid-Labile Linker Enables the Reuse of Solid Support for Fmoc-Based Solid Phase Synthesis.Macromolecular rapid communications · 2025Article
- Selective oxidative modification of tryptophan and cysteine residues using visible light responsive Rh doped SrTiOScientific reports · 2025Article
- Beyond Efficacy: Ensuring Safety in Peptide Therapeutics through Immunogenicity Assessment.Journal of peptide science : an official publication of the European Peptide Society · 2025Review
- Rapid synthesis of glycosylated insulins by flow-based peptide synthesis.Chemical science · 2025Article
- Fundamental Aspects of SPPS and Green Chemical Peptide Synthesis.Journal of peptide science : an official publication of the European Peptide Society · 2025Article
- Preparation, Modification, Food Application, and Health Effects of Protein and Peptide from Hemp (Foods (Basel, Switzerland) · 2025Review
- Ultra-Efficient Solid-Phase Peptide Synthesis.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Progress in the classification, optimization, activity, and application of antimicrobial peptides.Frontiers in microbiology · 2025Review
- Nanoparticles with Cell-Penetrating Peptides for Oral Delivery: A Case for Oral Delivery of Insulin.International journal of nanomedicine · 2025Review
- Recombinant and Synthetic Affibodies Function Comparably for Modulating Protein Release.Cellular and molecular bioengineering · 2024Article
- Enhanced native chemical ligation by peptide conjugation in trifluoroacetic acid.Science advances · 2024Article
- Synthesis and Hypoglycemic Effect of Insulin from the Venom of Sea AnemoneMarine drugs · 2024Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We present a process for solid phase peptide synthesis (SPPS) that completely eliminates all solvent intensive washing steps during each amino acid addition cycle. A key breakthrough is the removal of a volatile Fmoc deprotection base through bulk evaporation at elevated temperature while preventing condensation on the vessel surfaces with a directed headspace gas flushing. This process was demonstrated at both research and production scales without any impact on product quality and when applied to a variety of challenging sequences (up to 89 amino acids in length). The overall result is an extremely fast, high purity, scalable process with a massive waste reduction (up to 95%) while only requiring 10-15% of the standard amount of base used. This transformation of SPPS represents a step-change in peptide manufacturing process efficiency, and should encourage expanded access to peptide-based therapeutics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.