Evidence map›Paper›PMID 38070499›Full record

ArticleStructure (London, England : 1993)2024

The roles of FUS-RNA binding domain and low complexity domain in RNA-dependent phase separation.

Laura R Ganser, Amirhossein Ghanbari Niaki, Xincheng Yuan, Ethan Huang, Dahlia Deng, Nathalie A Djaja, Yingda Ge, Alanna Craig, Olivia Langlois, Sua Myong

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Structure (London, England : 1993), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
2.9field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 19 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Article
  6. Review
  7. Review
  8. RNA G-Quadruplexes Function as a Tunable Switch of FUS Phase Separation.bioRxiv : the preprint server for biology · 2025
    Article
  9. Article
  10. Review
  11. Article
  12. Article
  13. Review
  14. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Laura R GanserDepartment of Biophysics, Johns Hopkins University, Baltimore, MD 21218, USA.
Amirhossein Ghanbari NiakiDepartment of Biophysics, Johns Hopkins University, Baltimore, MD 21218, USA.
Xincheng YuanDepartment of Biophysics, Johns Hopkins University, Baltimore, MD 21218, USA; Program in Cell, Molecular, Developmental Biology, and Biophysics, Johns Hopkins University, 3400 N Charles St, Baltimore, MD 21218, USA; Department of Biology, Johns Hopkins University, 3400 N Charles St, Baltimore, MD 21218, USA.
Ethan HuangDepartment of Biophysics, Johns Hopkins University, Baltimore, MD 21218, USA.
Dahlia DengProgram in Cell, Molecular, Developmental Biology, and Biophysics, Johns Hopkins University, 3400 N Charles St, Baltimore, MD 21218, USA; Department of Biology, Johns Hopkins University, 3400 N Charles St, Baltimore, MD 21218, USA.
Nathalie A DjajaDepartment of Biophysics, Johns Hopkins University, Baltimore, MD 21218, USA; Program in Cell, Molecular, Developmental Biology, and Biophysics, Johns Hopkins University, 3400 N Charles St, Baltimore, MD 21218, USA; Department of Biology, Johns Hopkins University, 3400 N Charles St, Baltimore, MD 21218, USA.
Yingda GeDepartment of Biophysics, Johns Hopkins University, Baltimore, MD 21218, USA.
Alanna CraigDepartment of Biophysics, Johns Hopkins University, Baltimore, MD 21218, USA.
Olivia LangloisDepartment of Biophysics, Johns Hopkins University, Baltimore, MD 21218, USA.
Sua MyongDepartment of Biophysics, Johns Hopkins University, Baltimore, MD 21218, USA; Program in Cell, Molecular, Developmental Biology, and Biophysics, Johns Hopkins University, 3400 N Charles St, Baltimore, MD 21218, USA; Department of Biology, Johns Hopkins University, 3400 N Charles St, Baltimore, MD 21218, USA. Electronic address: smyong@jhu.edu.
Johns Hopkins University · US

Funding

Cellular and Molecular BiologyT32GM141804 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI John Kim · 2021 to 2026
$5.1M
Molecular Assembly and Disassembly of RNA-Protein Complexes Implicated in ALS/FTDRF1NS113636 · NINDS · JOHNS HOPKINS UNIVERSITY · PI MYONG, SUA · 2019 to 2019
$2.0M
Nucleation and Dissolution Mechanism Underlying ALS/FTLD-linked FUS CondensatesF31NS124267 · NINDS · JOHNS HOPKINS UNIVERSITY · PI DJAJA, NATHALIE ASHLEY · 2021 to 2023
$128k
The Role of RNA in Defining FUS Specificity and Activity in Phase Separation and SplicingF32GM139268 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI GANSER, LAURA R. · 2021 to 2022
$91k
NIGMS NIH HHS F32 GM139268NIGMS NIH HHS T32 GM141804NINDS NIH HHS F31 NS124267NINDS NIH HHS RF1 NS113636
6 · The paper itself

Abstract

Fused in sarcoma (FUS) is an archetypal phase separating protein asymmetrically divided into a low complexity domain (LCD) and an RNA binding domain (RBD). Here, we explore how the two domains contribute to RNA-dependent phase separation, RNA recognition, and multivalent complex formation. We find that RBD drives RNA-dependent phase separation but forms large and irregularly shaped droplets that are rescued by LCD in trans. Electrophoretic mobility shift assay (EMSA) and single-molecule fluorescence assays reveal that, while both LCD and RBD bind RNA, RBD drives RNA engagement and multivalent complex formation. While RBD alone exhibits delayed RNA recognition and a less dynamic RNP complex compared to full-length FUS, LCD in trans rescues full-length FUS activity. Likewise, cell-based data show RBD forms nucleolar condensates while LCD in trans rescues the diffuse nucleoplasm localization of full-length FUS. Our results point to a regulatory role of LCD in tuning the RNP interaction and buffering phase separation.

Indexed as

Phase SeparationRNARNA-Binding MotifsRNA-Binding Protein FUSHumansFUS protein, humanRNARNA-Binding Protein FUS

Identifiers

PMID38070499
PMCPMC10997494
OpenAlexW4389488960

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.