Evidence map›Paper›PMID 38070111›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Kinetic Detection of Apoptosis Events Via Caspase 3/7 Activation in a Tumor-Immune Microenvironment on a Chip.

Francesca Romana Bertani, Farnaz Dabbagh Moghaddam, Cristiano Panella, Sara Maria Giannitelli, Valentina Peluzzi, Annamaria Gerardino, Alberto Rainer, Giuseppe Roscilli, Adele De Ninno, Luca Businaro

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.0field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Francesca Romana BertaniCNR-IFN Institute for Photonics and Nanotechnologies, Rome, Italy.
Farnaz Dabbagh MoghaddamCNR-IFN Institute for Photonics and Nanotechnologies, Rome, Italy.
Cristiano PanellaUniversità Campus Bio-Medico di Roma, Rome, Italy.
Sara Maria GiannitelliUniversità Campus Bio-Medico di Roma, Rome, Italy.
Valentina PeluzziUniversità Campus Bio-Medico di Roma, Rome, Italy.
Annamaria GerardinoCNR-IFN Institute for Photonics and Nanotechnologies, Rome, Italy.
Alberto RainerUniversità Campus Bio-Medico di Roma, Rome, Italy.
Giuseppe RoscilliTakis s.r.l., Romano, Italy.
Adele De NinnoCNR-IFN Institute for Photonics and Nanotechnologies, Rome, Italy.
Luca BusinaroCNR-IFN Institute for Photonics and Nanotechnologies, Rome, Italy. luca.businaro@cnr.it.
Center for Biomolecular Nanotechnologies · ITUniversità Campus Bio-Medico · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The development of advanced biological models like microphysiological systems, able to rebuild the complexity of the physiological and/or pathological environments at a single-cell detail level in an in-vivo-like approach, is proving to be a promising tool to understand the mechanisms of interactions between different cell populations and main features of several diseases. In this frame, the tumor-immune microenvironment on a chip represents a powerful tool to profile key aspects of cancer progression, immune activation, and response to therapy in several immuno-oncology applications. In the present chapter, we provide a protocol to identify and characterize the time evolution of apoptosis by time-lapse fluorescence and confocal imaging in a 3D microfluidic coculture murine model including cancer and spleen cells.

Indexed as

NeoplasmsAnimalsApoptosisCaspase 3HumansLab-On-A-Chip DevicesMiceMicrofluidicsTumor MicroenvironmentCaspase 3ApoptosisCancerFluorescence microscopyMicroenvironmentMicrofluidicsMicro-physiological systemsOrgans-on-chipTimelapse imaging

Identifiers

PMID38070111
OpenAlexW4389507994

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.