Evidence map›Paper›PMID 38070078›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Mechanical Ablation of Larval Zebrafish Spinal Cord.

Samuel Henry Crossman, Mitra Amiri Khabooshan, Sebastian-Alexander Stamatis, Celia Vandestadt, Jan Kaslin

Erratum issuedAbstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors.

Samuel Henry CrossmanThe Australian Regenerative Medicine Institute, Monash University, Clayton, VIC, Australia. samuel.crossman@monash.edu.
Mitra Amiri KhabooshanThe Australian Regenerative Medicine Institute, Monash University, Clayton, VIC, Australia.
Sebastian-Alexander StamatisThe Australian Regenerative Medicine Institute, Monash University, Clayton, VIC, Australia.
Celia VandestadtThe Australian Regenerative Medicine Institute, Monash University, Clayton, VIC, Australia.
Jan KaslinThe Australian Regenerative Medicine Institute, Monash University, Clayton, VIC, Australia. jan.kaslin@monash.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Unlike mammals, adult and larval zebrafish exhibit robust regeneration following traumatic spinal cord injury. This remarkable regenerative capacity, combined with exquisite imaging capabilities and an abundance of powerful genetic techniques, has established the zebrafish as an important vertebrate model for the study of neural regeneration. Here, we describe a protocol for the complete mechanical ablation of the larval zebrafish spinal cord. With practice, this protocol can be used to reproducibly injure upward of 100 samples per hour, facilitating the high-throughput screening of factors involved in spinal cord regeneration and repair.

Indexed as

Spinal Cord InjuriesSpinal Cord RegenerationAnimalsLarvaMammalsNerve RegenerationSpinal CordZebrafishRegenerationSpinal cord injuryTungsten needle fabricationZebrafish

Identifiers

PMID38070078

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.