Evidence map›Paper›PMID 38070002›Full record

ArticleArchives of microbiology2023

Altering Escherichia coli envelope integrity by mimicking the lipoprotein RcsF.

Moustafa A TagElDein, Noha G Mohamed, Yasser E Shahein, Laila Ziko, Nahla A Hussein

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Archives of microbiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Moustafa A TagElDein *Microbiology and Immunology Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt.ORCID http://orcid.org/0000-0002-0119-0534
Noha G Mohamed *Pharmaceutical Chemistry Department, Faculty of Pharmacy, Sphinx University, Assiut, Egypt.ORCID http://orcid.org/0000-0003-1010-6979
Yasser E ShaheinMolecular Biology Department, Biotechnology Research Institute, National Research Centre, Cairo, Egypt.ORCID http://orcid.org/0000-0002-8886-3227
Laila ZikoDepartment of Biochemistry, School of Life and Medical Sciences, University of Hertfordshire Hosted By the Global Academic Foundation, R5 New Garden City, New Administrative Capital, Cairo, Egypt.ORCID http://orcid.org/0000-0003-1364-8255
Nahla A HusseinMolecular Biology Department, Biotechnology Research Institute, National Research Centre, Cairo, Egypt. Nahlahussein@aucegypt.edu.ORCID http://orcid.org/0000-0003-2520-0896
National Research Centre · EGCairo University · EGSphinx University · EG

Funding

Academy of Scientific Research and Technology, Egypt 57/2020
6 · The paper itself

Abstract

Escherichia coli cell envelope is crucial for stress sensing and signal transduction, mediated by numerous protein-protein interactions to enable adaptation and survival. Interfering with these interactions might affect envelope integrity leading to bacterial death. The outer membrane lipoprotein (RcsF) is the stress sensor of the regulator of capsule synthesis (Rcs) phosphorelay that senses envelope threats. RcsF interacts with two essential proteins, IgaA (repressing the Rcs system) and BamA (inserting β-barrel proteins in the outer membrane). Disturbing RcsF interactions may alter Rcs signaling and/or membrane integrity thus affecting bacterial survival. Here, we derived the sequence of a peptide mimicking RcsF (RcsFmim), based on the in silico docking of RcsF with IgaA. Expression of rcsFmim caused 3-to-4-fold activation of the Rcs system and perturbation of the outer membrane. Both effects result in decreased E. coli growth rate. We anticipate that RcsFmim present a candidate for future antibacterial peptide development.

Indexed as

Escherichia coliEscherichia coli ProteinsBacterial Outer Membrane ProteinsLipoproteinsPeptidesBacterial Outer Membrane ProteinsBamA protein, E coliEscherichia coli ProteinsLipoproteinsPeptidesAntibacterialAntimicrobial peptideRcsFRcs system

Identifiers

PMID38070002
PMCPMC10710380
OpenAlexW4389507552

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.