Evidence map›Paper›PMID 38067131›Full record

ArticleCells2023

Quantification of the Culture Stability of Stem Cell Fractions from Oral-Derived, Human Mesenchymal Stem Cell Preparations: A Significant Step toward the Clinical Translation of Cell Therapies.

Hitesh Chopra, Chen Cao, Celia Sommer, Alex Dahlkemper, James Sugai, James L Sherley, Darnell Kaigler

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cells, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.6field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Hitesh ChopraKaigler Lab of Stem Cell Science and Tissue Regeneration, Department of Periodontics and Oral Medicine, School of Dentistry, University of Michigan, Ann Arbor, MI 48109, USA.ORCID 0000-0002-4125-9946
Chen CaoKaigler Lab of Stem Cell Science and Tissue Regeneration, Department of Periodontics and Oral Medicine, School of Dentistry, University of Michigan, Ann Arbor, MI 48109, USA.ORCID 0000-0001-7717-0882
Celia SommerKaigler Lab of Stem Cell Science and Tissue Regeneration, Department of Periodontics and Oral Medicine, School of Dentistry, University of Michigan, Ann Arbor, MI 48109, USA.
Alex DahlkemperKaigler Lab of Stem Cell Science and Tissue Regeneration, Department of Periodontics and Oral Medicine, School of Dentistry, University of Michigan, Ann Arbor, MI 48109, USA.
James SugaiKaigler Lab of Stem Cell Science and Tissue Regeneration, Department of Periodontics and Oral Medicine, School of Dentistry, University of Michigan, Ann Arbor, MI 48109, USA.
James L SherleyAsymmetrex®, LLC, Boston, MA 02130, USA.ORCID 0000-0002-9711-3751
Darnell KaiglerKaigler Lab of Stem Cell Science and Tissue Regeneration, Department of Periodontics and Oral Medicine, School of Dentistry, University of Michigan, Ann Arbor, MI 48109, USA.ORCID 0000-0003-3728-4310
University of Michigan · US

Funding

Mechanisms of dental pulp stem cell differentiation into functional endotheliumR01DE021410 · NIDCR · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI NOR, JACQUES EDUARDO · 2011 to 2021
$3.2M
Redefining mesenchymal stem cells: using their cellular and molecular phenotypes to determine their regenerative and therapeutic propertiesR01DE028657 · NIDCR · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI KAIGLER, DARNELL · 2019 to 2023
$2.1M
Increased Validation of a First Method for Counting Tissue Stem Cells SpecificallyR43HL154900 · NHLBI · ASYMMETREX, LLC · PI SHERLEY, JAMES L · 2020 to 2020
$420k
NHLBI NIH HHS R43 HL154900NIDCR NIH HHS R01 DE028657
6 · The paper itself

Abstract

A continuing limitation and major challenge in the development and utilization of predictable stem cell therapies (SCTs) is the determination of the optimal dosages of stem cells. Herein, we report the quantification of stem cell fractions (SCF) of human mesenchymal stem cell (MSC) preparations derived from oral tissues. A novel computational methodology, kinetic stem cell (KSC) counting, was used to quantify the SCF and specific cell culture kinetics of stem cells in oral alveolar bone-derived MSC (aBMSCs) from eight patients. These analyses established, for the first time, that the SCF within these heterogeneous, mixed-cell populations differs significantly among donors, ranging from 7% to 77% (ANOVA

Indexed as

Mesenchymal Stem CellsCell Culture TechniquesHumansSignal TransductionStem Cellsalveolar bonecell biomanufacturingkinetic stem cell countingmesenchymal stem cellsstem cell therapy

Identifiers

PMID38067131
PMCPMC10705797
OpenAlexW4389056838

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.