Evidence map›Paper›PMID 38064040›Full record

ArticleBiotechnology letters2024

Immobilization of rough morphotype Mycolicibacterium neoaurum R for androstadienedione production.

Anqi Zhao, Yamei Li, Lixia Wu, Zhi Wang, Yongkun Lv, Wenlong Xiong, Mohammad Asraful Alam, Guohua Liu, Jingliang Xu

Abstract read
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In one paragraph

Article in Biotechnology letters, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Anqi ZhaoSchool of Life Sciences, Zhengzhou University, Zhengzhou, 450001, Henan, China.
Yamei LiSchool of Life Sciences, Zhengzhou University, Zhengzhou, 450001, Henan, China.
Lixia WuSchool of Life Sciences, Zhengzhou University, Zhengzhou, 450001, Henan, China.
Zhi WangSchool of Chemical Engineering, Zhengzhou University, Zhengzhou, 450001, Henan, China.
Yongkun LvSchool of Chemical Engineering, Zhengzhou University, Zhengzhou, 450001, Henan, China.
Wenlong XiongSchool of Chemical Engineering, Zhengzhou University, Zhengzhou, 450001, Henan, China.
Mohammad Asraful AlamSchool of Chemical Engineering, Zhengzhou University, Zhengzhou, 450001, Henan, China.
Guohua LiuKey Laboratory of Feed Biotechnology, The Ministry of Agriculture and Rural Affairs of the People's Republic of China, Beijing, 100081, China.
Jingliang XuSchool of Chemical Engineering, Zhengzhou University, Zhengzhou, 450001, Henan, China. xujl@zzu.edu.cn.ORCID http://orcid.org/0000-0003-0029-5710
Zhengzhou University · CNMinistry of Agriculture and Rural Affairs · CN

Funding

Henan Provincial Key Research and Development Program 212102310865Innovation Leadership Program in Sciences and Technologies for Central Plains Talent Plan 214200510009Key Program of Henan Provincial Science and Technology R&D Plan Joint Fund for Cultivation of Superior Disciplines 222301420008National Natural Science Foundation of China 22078308National Natural Science Foundation of China 31800034Program for Science & Technology Innovative Research Team in the University of Henan Province 22IRTSTHN007
6 · The paper itself

Abstract

objectivesEnhance the androstadienedione (Androst-1,4-diene-3,17-dione, ADD) production of rough morphotype Mycolicibacterium neoaurum R by repeated-batch fermentation of immobilized cells.

resultsM. neoaurum R was a rough colony morphotype variant, obtained from the routine plating of smooth M. neoaurum strain CICC 21097. M. neoaurum R showed rougher cell surface and aggregated in broth. The ADD production of M. neoaurum R was notably lower than that of M. neoaurum CICC 21097 during the free cell fermentation, but the yield gap could be erased after proper cell immobilization. Subsequently, repeated-batch fermentation of immobilized M. neoaurum R was performed to shorten the production cycle and enhance the bio-production efficiency of ADD. Through the optimization of the immobilization carriers and the co-solvents for phytosterols, the ADD productivity of M. neoaurum R immobilized by semi-expanded perlite reached 0.075 g/L/h during the repeated-batch fermentation for 40 days.

conclusionsThe ADD production of the rough-type M. neoaurum R was notably enhanced by the immobilization onto semi-expanded perlite. Moreover, the ADD batch yields of M. neoaurum R immobilized by semi-expanded perlite were maintained at high levels during the repeated-batch fermentation.

Indexed as

MycobacteriaceaePhytosterolsSilicon DioxideAluminum OxideAluminum OxidePerlitePhytosterolsSilicon DioxideAndrost-1,4-diene-3,17-dioneCell immobilizationMycolicibacteriumPerliteRough colony morphotype variant

Identifiers

PMID38064040
OpenAlexW4389482692

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.