Evidence map›Paper›PMID 38064028›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Rescue of Infectious Salmon Anemia Virus (ISAV) from Cloned cDNA.

Daniela Toro-Ascuy, Matías Cárdenas, Yesseny Vásquez-Martínez, Marcelo Cortez-San Martín

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
2.2field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 2 countries.

Daniela Toro-AscuyLaboratory of Virology, Department of Biology, Faculty of Sciences, Universidad de Chile, Santiago, Chile.
Matías CárdenasDepartment of Population Health College of Veterinary Medicine, University of Georgia, Athens, GA, USA.
Yesseny Vásquez-MartínezEscuela de Medicina, Faculty of Health Sciences, University of Santiago de Chile, Santiago, Chile.
Marcelo Cortez-San MartínLaboratory of Molecular Virology and Pathogens Control, Faculty of Chemistry and Biology, University of Santiago de Chile, Santiago, Chile. marcelo.cortez@usach.cl.
Universidad de Santiago de Chile · CLUniversity of Chile · CLUniversity of Georgia · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The piscine orthomyxovirus called infectious salmon anemia virus (ISAV) is one of the most important emerging pathogens affecting the salmon industry worldwide. The first reverse genetics system for ISAV, which allows the generation of recombinant ISA virus (rISAV), is an important tool for the characterization and study of this virus. The plasmid-based reverse genetics system for ISAV includes the use of a novel fish promoter, the Atlantic salmon internal transcribed spacer region 1 (ITS-1). The salmon, viral, and mammalian genetic elements included in the pSS-URG vectors allow the expression of the eight viral RNA segments. In addition to four cytomegalovirus (CMV)-based vectors that express the four proteins of the ISAV ribonucleoprotein complex, the eight pSS-URG vectors allowed the generation of infectious rISAV in salmon cells.

Indexed as

Fish DiseasesIsavirusOrthomyxoviridaeOrthomyxoviridae InfectionsAnimalsCell LineDNA, ComplementaryMammalsRNA, ViralSalmonDNA, ComplementaryRNA, ViralInfectious salmon anemia virusISAVITS-1Reverse geneticsRNA polymerase IRNA polymerase IISalmon cellsTransfectionViral RNA

Identifiers

PMID38064028
OpenAlexW4389483469

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.