Evidence map›Paper›PMID 38062493›Full record

ArticleVirology journal2023

Identification of the protein coding capability of coronavirus defective viral genomes by mass spectrometry.

Ching-Hung Lin, Feng-Cheng Hsieh, Chien-Chen Lai, Wei-Chen Wang, Cheng-Yu Kuo, Chun-Chun Yang, Hsuan-Wei Hsu, Hon-Man-Herman Tam, Cheng-Yao Yang, Hung-Yi Wu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Virology journal, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.8field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Ching-Hung LinGraduate Institute of Veterinary Pathobiology, College of Veterinary Medicine, National Chung Hsing University, Taichung, 40227, Taiwan.
Feng-Cheng HsiehGraduate Institute of Veterinary Pathobiology, College of Veterinary Medicine, National Chung Hsing University, Taichung, 40227, Taiwan.
Chien-Chen LaiInstitute of Molecular Biology, College of Life Sciences, National Chung Hsing University, Taichung, 40227, Taiwan.
Wei-Chen WangInstitute of Molecular Biology, College of Life Sciences, National Chung Hsing University, Taichung, 40227, Taiwan.
Cheng-Yu KuoInstitute of Molecular Biology, College of Life Sciences, National Chung Hsing University, Taichung, 40227, Taiwan.
Chun-Chun YangGraduate Institute of Veterinary Pathobiology, College of Veterinary Medicine, National Chung Hsing University, Taichung, 40227, Taiwan.
Hsuan-Wei HsuGraduate Institute of Veterinary Pathobiology, College of Veterinary Medicine, National Chung Hsing University, Taichung, 40227, Taiwan.
Hon-Man-Herman TamDepartment of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, Taichung, 40227, Taiwan.
Cheng-Yao YangGraduate Institute of Veterinary Pathobiology, College of Veterinary Medicine, National Chung Hsing University, Taichung, 40227, Taiwan. yangchengyao@dragon.nchu.edu.tw.
Hung-Yi WuGraduate Institute of Veterinary Pathobiology, College of Veterinary Medicine, National Chung Hsing University, Taichung, 40227, Taiwan. hwu2@dragon.nchu.edu.tw.
National Chung Hsing University · TW

Funding

National Science and Technology Council 109-2313-B-005 -013 -MY3, 110-2327-B-005 -003 and 111-2327-B-005 -003
6 · The paper itself

Abstract

During coronavirus infection, in addition to the well-known coronavirus genomes and subgenomic mRNAs, an abundance of defective viral genomes (DVGs) can also be synthesized. In this study, we aimed to examine whether DVGs can encode proteins in infected cells. Nanopore direct RNA sequencing and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis were employed. With the protein databases generated by nanopore direct RNA sequencing and the cell lysates derived from the RNA-protein pull-down assay, six DVG-encoded proteins were identified by LC-MS/MS based on the featured fusion peptides caused by recombination during DVG synthesis. The results suggest that the coronavirus DVGs have the capability to encode proteins. Consequently, future studies determining the biological function of DVG-encoded proteins may contribute to the understanding of their roles in coronavirus pathogenesis and the development of antiviral strategies.

Indexed as

CoronavirusCoronavirus InfectionsChromatography, LiquidGenome, ViralHumansProteinsRNA, ViralTandem Mass SpectrometryProteinsRNA, ViralCoronavirusDefective viral genomeGene expressionPathogenesisProtein coding

Identifiers

PMID38062493
PMCPMC10704767
OpenAlexW4389430496

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.