Evidence map›Paper›PMID 38062348›Full record

ArticleBMC plant biology2023

Karyotyping of aneuploid and polyploid plants from low coverage whole-genome resequencing.

Kanglu Zhao, Yanbo Bai, Qingyu Zhang, Zhen Zhao, Yao Cao, Lu Yang, Ni Wang, Junxiong Xu, Bo Wang, Lei Wu and 7 more

Open access · goldAbstract read
In one paragraph

Article in BMC plant biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
3.2field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 8 citations in OpenAlex.

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  6. Targeted mutation ofHorticulture research · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors at 2 institutions in 1 country.

Kanglu ZhaoKey Laboratory of Herbage and Endemic Crop Biology, Ministry of Education, School of Life Sciences, Inner Mongolia University, Hohhot, 010070, China.
Yanbo BaiKey Laboratory of Herbage and Endemic Crop Biology, Ministry of Education, School of Life Sciences, Inner Mongolia University, Hohhot, 010070, China.
Qingyu ZhangKey Laboratory of Herbage and Endemic Crop Biology, Ministry of Education, School of Life Sciences, Inner Mongolia University, Hohhot, 010070, China.
Zhen ZhaoKey Laboratory of Herbage and Endemic Crop Biology, Ministry of Education, School of Life Sciences, Inner Mongolia University, Hohhot, 010070, China.
Yao CaoKey Laboratory of Herbage and Endemic Crop Biology, Ministry of Education, School of Life Sciences, Inner Mongolia University, Hohhot, 010070, China.
Lu YangKey Laboratory of Herbage and Endemic Crop Biology, Ministry of Education, School of Life Sciences, Inner Mongolia University, Hohhot, 010070, China.
Ni WangKey Laboratory of Herbage and Endemic Crop Biology, Ministry of Education, School of Life Sciences, Inner Mongolia University, Hohhot, 010070, China.
Junxiong XuKey Laboratory of Herbage and Endemic Crop Biology, Ministry of Education, School of Life Sciences, Inner Mongolia University, Hohhot, 010070, China.
Bo WangKey Laboratory of Herbage and Endemic Crop Biology, Ministry of Education, School of Life Sciences, Inner Mongolia University, Hohhot, 010070, China.
Lei WuKey Laboratory of Herbage and Endemic Crop Biology, Ministry of Education, School of Life Sciences, Inner Mongolia University, Hohhot, 010070, China.
Xiufeng GongKey Laboratory of Herbage and Endemic Crop Biology, Ministry of Education, School of Life Sciences, Inner Mongolia University, Hohhot, 010070, China.
Tuanrong LinInstitute of Ulanqab Agricultural and Forestry Sciences, Inner Mongolia, Ulanqab, 012000, China.
Yufeng WangInstitute of Ulanqab Agricultural and Forestry Sciences, Inner Mongolia, Ulanqab, 012000, China.
Wei WangInstitute of Ulanqab Agricultural and Forestry Sciences, Inner Mongolia, Ulanqab, 012000, China.
Xingkui CaiKey Laboratory of Horticultural Plant Biology, Key Laboratory of Potato Biology and Biotechnology, Ministry of Agriculture and Rural Affairs, College of Horticulture and Forestry Sciences, Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, China.
Yuhe YinInstitute of Ulanqab Agricultural and Forestry Sciences, Inner Mongolia, Ulanqab, 012000, China.
Zhiyong XiongKey Laboratory of Herbage and Endemic Crop Biology, Ministry of Education, School of Life Sciences, Inner Mongolia University, Hohhot, 010070, China. xiongzy2003@aliyun.com.
Inner Mongolia University · CNMinistry of Agriculture and Rural Affairs · CN

Funding

National Natural Science Foundation of China 32060503National Natural Science Foundation of China 32070556
6 · The paper itself

Abstract

backgroundKaryotype, as a basic characteristic of species, provides valuable information for fundamental theoretical research and germplasm resource innovation. However, traditional karyotyping techniques, including fluorescence in situ hybridization (FISH), are challenging and low in efficiency, especially when karyotyping aneuploid and polyploid plants. The use of low coverage whole-genome resequencing (lcWGR) data for karyotyping was explored, but existing methods are complicated and require control samples.

resultsIn this study, a new protocol for molecular karyotype analysis was provided, which proved to be a simpler, faster, and more accurate method, requiring no control. Notably, our method not only provided the copy number of each chromosome of an individual but also an accurate evaluation of the genomic contribution from its parents. Moreover, we verified the method through FISH and published resequencing data.

conclusionsThis method is of great significance for species evolution analysis, chromosome engineering, crop improvement, and breeding.

Indexed as

AneuploidyPolyploidyIn Situ Hybridization, FluorescenceKaryotypeKaryotypingAneuploidFISHKaryotypePolyploidWhole-genome resequencing

Identifiers

PMID38062348
PMCPMC10704825
OpenAlexW4389461960

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.