ArticleAnalytical chemistry2023
Lipidomic Phenotyping Of Human Small Intestinal Organoids Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging.
Article in Analytical chemistry, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 14 citations in OpenAlex.
- A Fully Defined GelMA-Based Matrix Allows Fine Tuning of Tissue-Relevant Biomechanical and Biochemical Cues for Organoid Culture.Advanced healthcare materials · 2026Article
- Miniaturized chromatography-based lipidomics methods towards single-cell analysis.Analytical and bioanalytical chemistry · 2026Review
- Dry Deposition Strategies for MALDI-MS Imaging: Principles, Advances and Emerging Applications.Analytical science advances · 2026Review
- MALDI spatial proteomics: a mini review of approaches and techniques.Analytical methods : advancing methods and applications · 2026Review
- From Sample to Mixed Reality: A Translational 3D MALDI Imaging Platform for Advanced 3D Spatial Omics Analysis of 3D Cell Culture Disease Models.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Filtration-Based Rapid ECM-Hydrogel Elimination Enables Robust MALDI MSI of Intestinal Organoids.Analytical chemistry · 2026Article
- Seeing between the lines: matrix-assisted laser desorption/ionization mass spectrometry imaging advances for microbial interactions.Current opinion in microbiology · 2025Review
- Imaging and spatially resolved mass spectrometry applications in nephrology.Nature reviews. Nephrology · 2025Review
- Spatially resolved metabolomics: From metabolite mapping to function visualising.Clinical and translational medicine · 2024Review
- Mass Spectrometry Imaging and Histology for the Analysis of Budding Intestinal Organoids.Analytical chemistry · 2024Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 3 countries.
Funding
Abstract
In the past decade, interest in organoids for biomedical research has surged, resulting in a higher demand for advanced imaging techniques. Traditional specimen embedding methods pose challenges, such as analyte delocalization and histological assessment. Here, we present an optimized sample preparation approach utilizing an Epredia M-1 cellulose-based embedding matrix, which preserves the structural integrity of fragile small intestinal organoids (SIOs). Additionally, background interference (delocalization of analytes, nonspecific (histological) staining, matrix ion clusters) was minimized, and we demonstrate the compatibility with matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI). With our approach, we can conduct label-free lipid imaging at the single-cell level, thereby yielding insights into the spatial distribution of lipids in both positive and negative ion modes. Moreover, M-1 embedding allows for an improved coregistration with histological and immunohistochemical (IHC) stainings, including MALDI-IHC, facilitating combined untargeted and targeted spatial information. Applying this approach, we successfully phenotyped crypt-like (CL) and villus-like (VL) SIOs, revealing that PE 36:2 [M - H]
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.